Professor Chris Toseland

School of Medicine and Population Health

Professor of Cancer Biophysics

Chris Toseland
Profile picture of Chris Toseland
c.toseland@sheffield.ac.uk
+44 114 215 9080
FU34, F Floor, The Medical School

Full contact details

Professor Chris Toseland
School of Medicine and Population Health
FU34, F Floor
The Medical School
Beech Hill Road
Sheffield
S10 2RX
Profile

For enquiries please contact - SMPH-West-Operational@sheffield.ac.uk

2019: Senior Lecturer/MRC Career Development Award Fellowship
School of Medicine & Population Health - University of Sheffield

2015 - 2019: MRC Career Development Award
Schools of Biosciences - University of Kent

April 2015: Leverhulme Early Career Research Fellow
Schools of Biosciences - University of Kent

2013 – 2015: Senior Post-Doc Researcher
Max-Planck Institute for Biochemistry, Munich, Germany

2010 – 2013: EMBO Long Term Fellow
LMU Munich, Munich, Germany

2009 – 2010: Post-Doctoral Research Associate
MRC National Institute for Medical Research, London, UK

2006 – 2009: PhD
MRC National Institute for Medical Research, London, UK

2002 – 2006: BSc Biochemistry
University of Wales - Aberystwyth

Research interests

The lab specialises in application of fluorescence and mechanical measurements from whole cells to single molecules. We are a multidisciplinary lab drawing upon core techniques in Cell Biology, Biochemistry, Biophysics, Mechanobiology, Super Resolution single molecule imaging and Genomics Gene expression, the transfer of the genetic code into cellular proteins is one of the most fundamental processes in living cells. This process is orchestrated by RNA polymerases, which are highly regulated to ensure correct expression.

A breakdown in this regulation leads to development disabilities and most notably cancer formation. Furthermore, changes in expression control embryonic development and stem cell differentiation.

Over the past decade it has become established that myosin motors are present in the nucleus and function in transcription, but what role they play in the organisation and transcription of genetic information is unclear.

For the first time we combine classical biochemical and single molecule assays to provide a quantitative high-resolution description of the activity of these nuclear myosins; providing a mechanistic insight into the activity of these proteins.

Please see External Lab HomePage for more information.

Publications

Show: Featured publications All publications

Journal articles

  • Santos ÁD, Fili N, Pearson DS, Hari-Gupta Y & Toseland CP (2021) . Biophysical Journal, 120(4), 631-641.
  • dos Santos A, Cook A, Gough R, Schilling M, Aleida Olszok N, Brown I, Wang L, Aaron J, Martin-Fernandez M, Rehfeldt F & Toseland C (2021) . Nucleic Acids Research, 49(1), 340-353.
  • Cook AW, Gough RE & Toseland CP (2020) . Journal of Cell Science, 133(11).
  • Fili N, Hari-Gupta Y, Aston B, dos Santos Á, Gough RE, Alamad B, Wang L, Martin-Fernandez ML & Toseland CP (2020) . Journal of Biological Chemistry, 295(2), 337-347.
  • Acton SE, Bell AJD, Toseland CP & Twelvetrees A (2019) . eLife, 2019(8).
  • Fili N, Hari-Gupta Y, dos Santos Á, Cook A, Poland S, Ameer-Beg SM, Parsons M & Toseland CP (2017) . Nature Communications, 8(-), ---.
  • Lherbette M, dos Santos Á, Hari-Gupta Y, Fili N, Toseland CP & Schaap IAT (2017) . Scientific Reports, 7.
  • Fili N & Toseland CP () . International Journal of Molecular Sciences, 21(1), 67-67.

All publications

Journal articles

  • Gawor M, Lehka L, Lambert D & Toseland CP (2025) . Journal of Cell Science, 138(3).
  • dos Santos A, Rollins DE, Hari-Gupta Y, Reed HCW, Du M, Ru SYZ, Pidlisna K, Stranger A, Lorgat F, Lambert D , Brown I et al (2023) . Nature Communications, 14.
  • Shahid-Fuente IW & Toseland CP (2023) . Biochemical Society Transactions, 51(3), 1023-1034.
  • dos Santos Á, Fili N, Hari-Gupta Y, Gough RE, Wang L, Martin-Fernandez M, Aaron J, Wait E, Chew T-L & Toseland CP (2022) . Biochemical Journal, 479(13), 1409-1428.
  • dos Santos Á, Rehfeldt F & Toseland CP (2022) , 171-181.
  • dos Santos Á, Gough RE, Wang L & Toseland CP (2022) , 293-309.
  • Hari-Gupta Y, Fili N, dos Santos Á, Cook AW, Gough RE, Reed HCW, Wang L, Aaron J, Venit T, Wait E , Grosse-Berkenbusch A et al (2022) . Nature Communications, 13(1).
  • dos Santos A & Toseland C (2022) . Journal of Visualized Experiments, 180.
  • dos Santos Á & Toseland CP (2021) . International Journal of Molecular Sciences, 22(6).
  • Cook AW & Toseland CP (2021) . The Journal of Biochemistry, 169(3), 265-271.
  • Santos ÁD, Fili N, Pearson DS, Hari-Gupta Y & Toseland CP (2021) . Biophysical Journal, 120(4), 631-641.
  • Emmanouilidis I, Fili N, Cook AW, Hari-Gupta Y, dos Santos Á, Wang L, Martin-Fernandez ML, Ellis PJI & Toseland CP (2021) . Biomolecules, 11(2).
  • dos Santos A, Cook A, Gough R, Schilling M, Aleida Olszok N, Brown I, Wang L, Aaron J, Martin-Fernandez M, Rehfeldt F & Toseland C (2021) . Nucleic Acids Research, 49(1), 340-353.
  • Cook AW, Gough RE & Toseland CP (2020) . Journal of Cell Science, 133(11).
  • Fili N, Hari-Gupta Y, Aston B, dos Santos Á, Gough RE, Alamad B, Wang L, Martin-Fernandez ML & Toseland CP (2020) . Journal of Biological Chemistry, 295(2), 337-347.
  • Acton SE, Bell AJD, Toseland CP & Twelvetrees A (2019) . eLife, 2019(8).
  • Fili N, Hari-Gupta Y, Aston B, dos Santos Á, Gough RE, Alamad B, Wang L, Martin-Fernandez M & Toseland CP (2019) .
  • Cook A, Hari-Gupta Y & Toseland CP (2018) . Biochemical and Biophysical Research Communications, 496(3), 820-825.
  • Fili N, Hari-Gupta Y, dos Santos Á, Cook A, Poland S, Ameer-Beg SM, Parsons M & Toseland CP (2017) . Nature Communications, 8(-), ---.
  • Toseland C (2017) . Impact, 2017(10), 49-51.
  • Lherbette M, dos Santos Á, Hari-Gupta Y, Fili N, Toseland CP & Schaap IAT (2017) . Scientific Reports, 7.
  • Cook A, Hari-Gupta Y & Toseland CP (2017) .
  • Yao M, Goult BT, Klapholz B, Hu X, Toseland CP, Guo Y, Cong P, Sheetz MP & Yan J (2016) . Nature Communications, 7.
  • Wilhelm L, Buermann F, Minnen A, Shin H-C, Toseland CP, Oh B-H & Gruber S (2015) . eLife, 4.
  • Kang H-A, Shin H-C, Kalantzi A-S, Toseland CP, Kim H-M, Gruber S, Dal Peraro M & Oh B-H (2015) . Nucleic Acids Research, 43(7), 3841-3856.
  • Soh Y-M, Buermann F, Shin H-C, Oda T, Jin KS, Toseland CP, Kim C, Lee H, Kim SJ, Kong M-S , Durand-Diebold M-L et al (2015) . Molecular Cell, 57(2), 290-303.
  • Toseland CP & Geeves MA (2014) , 49-65.
  • Fili N & Toseland CP (2014) , 1-24.
  • Toseland CP (2014) , 67-86.
  • Toseland CP & Webb MR (2013) . Journal of Biological Chemistry, 288(35), 25183-25193.
  • Toseland CP (2013) . Journal of Chemical Biology, 6(3), 85-95.
  • Chisty LT, Toseland CP, Fili N, Mashanov GI, Dillingham MS, Molloy JE & Webb MR (2013) . Nucleic Acids Research, 41(9), 5010-5023.
  • Batters C, Woodall KA, Toseland CP, Hundschell C & Veigel C (2012) . Journal of Biological Chemistry, 287(33), 27556-27566.
  • Batters C, Woodall K, Toseland C & Veigel C (2012) . Biophysical Journal, 102(3), 570a-570a.
  • Toseland CP & Webb MR (2011) , 161-174.
  • Fili N, Toseland CP, Dillingham MS, Webb MR & Molloy JE (2011) , 193-214.
  • Fili N, Mashanov GI, Toseland CP, Batters C, Wallace MI, Yeeles JTP, Dillingham MS, Webb MR & Molloy JE (2010) . Nucleic Acids Research, 38(13), 4448-4457.
  • Toseland CP & Webb MR (2010) . Methods, 51(3), 259-268.
  • Toseland CP, Martinez-Senac MM, Slatter AF & Webb MR (2009) . Journal of Molecular Biology, 392(4), 1020-1032.
  • Davies MN, Guan P, Blythe MJ, Salomon J, Toseland CP, Hattotuwagama C, Walshe V, Doytchinova IA & Flower DR (2007) . Expert Opinion on Drug Discovery, 2(1), 19-35.
  • Taylor PD, Toseland CP, Attwood TK & Flower DR (2006) Alpha helical trans-membrane proteins: Enhanced prediction using a Bayesian approach.. Bioinformation, 1(6), 234-236.
  • Taylor PD, Toseland CP, Attwood TK & Flower DR (2006) A predictor of membrane class: Discriminating alpha-helical and beta-barrel membrane proteins from non-membranous proteins.. Bioinformation, 1(6), 208-213.
  • Taylor PD, Toseland CP, Attwood TK & Flower DR (2006) Beta barrel trans-membrane proteins: Enhanced prediction using a Bayesian approach.. Bioinformation, 1(6), 231-233.
  • Hattotuwagama CK, Toseland CP, Guan P, Taylor DJ, Hemsley SL, Doytchinova IA & Flower DR (2006) . ChemInform, 37(31).
  • Davies MN, Toseland CP, Moss DS & Flower DR (2006) . BMC Biochemistry, 7(1).
  • Toseland CP (2006) . Nucleic Acids Research, 34(90001), D199-D203.
  • Hattotuwagama CK, Toseland CP, Guan P, Taylor DJ, Hemsley SL, Doytchinova IA & Flower DR (2006) . Journal of Chemical Information and Modeling, 46(3), 1491-1502.
  • Toseland CP, Clayton DJ, McSparron H, Hemsley SL, Blythe MJ, Paine K, Doytchinova IA, Guan P, Hattotuwagama CK & Flower DR (2005) . Immunome Research, 1(1), 4-4.
  • Guan P, Davies M, Taylor D, Wan S, McSparron H, Hemsley S, Toseland C, Blythe M, Taylor P, Walshe V , Hattotuwagama C et al (2005) . Current Computer Aided-Drug Design, 1(4), 377-395.
  • Fili N & Toseland CP () . International Journal of Molecular Sciences, 21(1), 67-67.
  • Toseland CP, Powell B & Webb MR () . PLoS ONE, 7(6), e38270-e38270.
  • Taylor PD, Toseland CP, Attwood TK & Flower DR () . Bioinformation, 1(5), 184-187.
  • Taylor PD, Toseland CP, Attwood TK & Flower DR () . Bioinformation, 1(5), 176-179.
  • Toseland CP, McSparron HM & Flower DR () . BMC Bioinformatics, 6(1).

Book chapters

  • Webb MR & Toseland CP (2013) , Encyclopedia of Biophysics (pp. 950-958). Springer Berlin Heidelberg
  • Fili N, Toseland CP, Dillingham MS, Webb MR & Molloy JE (2011) , Methods in Molecular Biology (pp. E1-E1). Humana Press
  • Hattotuwagama CK, Guan P, Davies M, Taylor DJ, Walshe V, Hemsley SL, Toseland C, Doytchinova IA, Borrow P & Flower DR () , In Silico Immunology (pp. 139-175). Springer US

Conference proceedings

  • Cook A, Hari Gupta Y, Venit T, Percipalle P & Toseland CP (2019) . Biophysical Journal, Vol. 116(3) (pp 77a-77a)
  • dos Santos A, Wang L & Toseland CP (2019) . Biophysical Journal, Vol. 116(3) (pp 210a-210a)
  • Fili N, Cook A, Hari Gupta Y & Toseland CP (2019) . Biophysical Journal, Vol. 116(3) (pp 178a-178a)
  • Gough RE, Fili N, Hari Gupta Y & Toseland CP (2019) . Biophysical Journal, Vol. 116(3) (pp 260a-260a)
  • Hari Gupta Y, Fili N, dos Santos A, Chew T-L, Aaron J, Wang L & Toseland CP (2019) . Biophysical Journal, Vol. 116(3) (pp 208a-209a)
  • Hari Gupta Y, Fili N, dos Santos A, Chew T-L, Aaron J, Wang L & Toseland CP (2019) . Biophysical Journal, Vol. 116(3) (pp 209a-209a)
  • Fili N, dos Santos A, Hari-Gupta Y & Toseland CP (2016) Activation of nuclear myosin VI leads to enhanced gene expression.. MOLECULAR BIOLOGY OF THE CELL, Vol. 27
  • Fili N, Hari-Gupta Y & Toseland CP (2016) Dissecting the role of Myosin VI in the nucleus.. MOLECULAR BIOLOGY OF THE CELL, Vol. 27
  • Toseland CP, Ellrich H, Fili N, Batters C & Veigel C (2012) . Biophysical Journal, Vol. 102(3) (pp 569a-569a)
  • Veigel C, Batters C, Toseland CP & Sellers JR (2011) . Biophysical Journal, Vol. 100(3) (pp 116a-116a)
  • Toseland CP, Batters C & Veigel C (2011) . Biophysical Journal, Vol. 100(3) (pp 72a-73a)
  • Toseland CP & Webb MR (2009) . Biophysical Journal, Vol. 96(3) (pp 415a-415a)

Preprints

  • González-Novo R, Zamora-Carreras H, Lope-Planelles AD, López-Menéndez H, Roda-Navarro P, Monroy F, Wang L, Toseland CP & Redondo Muñoz J (2023) , Cold Spring Harbor Laboratory.
  • dos Santos Á, Rollins DE, Hari-Gupta Y, Reed HCW, Du M, Ru SYZ, Pidlisna K, Stranger A, Lorgat F, Brown I , Howland K et al (2022) , Cold Spring Harbor Laboratory.
  • Emmanouilidis I, Fili N, Cook AW, Hari-Gupta Y, dos Santos Á, Wang L, Martin-Fernandez M, Ellis PJI & Toseland CP (2021) , Cold Spring Harbor Laboratory.
  • dos Santos Á, Cook AW, Gough RE, Schilling M, Olszok NA, Brown I, Wang L, Aaron J, Martin-Fernandez ML, Rehfeldt F & Toseland CP (2020) , Cold Spring Harbor Laboratory.
  • dos Santos Á, Fili N, Hari-Gupta Y, Gough RE, Wang L, Martin-Fernandez M, Aaron J, Wait E, Chew T-L & Toseland CP (2020) , Cold Spring Harbor Laboratory.
  • Santos ÁD, Fili N, Pearson DS, Hari-Gupta Y & Toseland CP (2020) , Cold Spring Harbor Laboratory.
  • Hari-Gupta Y, Fili N, dos Santos Á, Cook AW, Gough RE, Reed HCW, Wang L, Aaron J, Venit T, Wait E , Grosse-Berkenbusch A et al (2020) , Cold Spring Harbor Laboratory.
  • Große-Berkenbusch A, Hettich J, Kuhn T, Fili N, Cook AW, Hari-Gupta Y, Palmer A, Streit L, Ellis PJI, Toseland CP & Gebhardt JCM (2020) , Cold Spring Harbor Laboratory.
Research group
  • Dr Natalia Fili (Postdoctoral Research Associate)
  • Rosemarie Gough (Postdoctoral Research Associate)
  • Alexander Cook (Postdoctoral Research Associate)
  • Alia dos Santos (PhD Student)
Grants
  • Medical Research Council
  • Cancer Research UK
  • Science and Technology Facilities Council
  • Royal Society
  • Leverhulme Trust