Dr Luke Green

School of Clinical Dentistry

Lecturer in Microbiology

Luke Green
Profile picture of Luke Green
l.r.green@sheffield.ac.uk
+44 114 215 9547
Room LU105, L Floor, 19 Claremont Crescent

Full contact details

Dr Luke Green
School of Clinical Dentistry
Room LU105, L Floor
19 Claremont Crescent
Sheffield
S10 2TA
Profile
  • 2025Գ: Lecturer in Microbiology; University of Sheffield, UK
  • 2021–2025: The Humane Research Trust Research Fellow; University of Sheffield, UK
  • 2018–2021: Postdoctoral Associate; Prof. Pete Monk, University of Sheffield, UK
  • 2015–2018: Postdoctoral Researcher; Dr. Chris Bayliss, University of Leicester, UK
  • 2011–2015: Research Associate; Dr. Ingrid Scully Group, Pfizer Inc., USA
  • 2006–2010: PhD; Prof. Rob Read Group, University of Sheffield, UK.
Research interests

Infectious disease is a huge health burden globally and antibiotic resistance is an ever growing problem, currently attributable to ~700,000 deaths per year with projected figures rising to 10 million by the year 2050.  For this reason, new therapies to replace antibiotics are crucial. Due to their rapid doubling time, bacteria have the ability to quickly evolve mechanisms to overcome drugs targeted to them, for this reason, I believe the future for anti-infectives is to target infection at the human cell level. Bacteria enter our cells by "hijacking" parts of our own cell machinery (proteins).

Our cells are encased in a membrane consisting of water-repelling fatty acids and proteins. Bacteria have to attach to and breach this membrane in order to get inside our cells. They do this by "hijacking" the proteins within the membrane. Whilst there has been extensive research into how bacteria bind our cells, no universal mechanisms (used by all bacteria) are known. Different bacterial species have been shown to hijack different proteins, which causes problems with therapeutic design. I have discovered that a family of human cell membrane proteins, the tetraspanins, are extremely important in the binding of multiple bacterial species to our cells. Targeting tetraspanins using drugs reduces bacterial binding to multiple types of human cells by greater than 50%. The tetraspanins form part of our cell membranes and act as "organisers", bringing together lots of other proteins to form large protein "islands" in the cell membrane. Bacteria do not directly attach to the tetraspanins but I hypothesise that the tetraspanins organize the proteins required for bacterial binding and it is therefore the loss of this organization which results in reduced bacterial adhesion to our cells when the tetraspanins are blocked.

My lab is interested in investigating the role of these proteins and their interactors in bacterial infection.

Publications

Show: Featured publications All publications

Journal articles

  • Jadi PK, Dave A, Issa R, Tabbasum K, Okurowska K, Samarth A, Urwin L, Green LR, Partridge LJ, MacNeil S , Garg P et al (2024) . The Ocular Surface, 32, 211-218.
  • Green LR, Issa R, Albaldi F, Urwin L, Thompson R, Khalid H, Turner CE, Ciani B, Partridge LJ & Monk PN (2023) . mBio, 14(4).
  • Wanford JJ, Holmes JC, Bayliss CD & Green LR (2020) . Microbial Genomics, 6(5).
  • Green LR, Al-Rubaiawi AA, Al-Maeni MARM, Harrison OB, Blades M, Oldfield NJ, Turner DPJ, Maiden MCJ & Bayliss CD (2020) . mBio, 11(2).
  • Green LR, Dave N, Adewoye AB, Lucidarme J, Clark SA, Oldfield NJ, Turner DPJ, Borrow R & Bayliss CD (2019) . The Journal of Infectious Diseases, 220(7), 1109-1117.
  • Green LR, Lucidarme J, Dave N, Chan H, Clark S, Borrow R & Bayliss CD (2018) . Journal of Clinical Microbiology, 56(9).
  • Oldfield NJ, Green LR, Parkhill J, Bayliss CD & Turner DPJ (2018) . The Journal of Infectious Diseases, 217(4), 608-616.
  • Green LR, Monk PN, Partridge LJ, Morris P, Gorringe AR & Read RC (2011) . Infect Immun, 79(6), 2241-2249.

Preprints

  • Wolverson PA, Fernandes Parreira I, Collins MO, Shaw JG & Green LR (2024) , Cold Spring Harbor Laboratory.

All publications

Journal articles

  • Wolverson PA, Fernandes Parreira I, Thompson RH, Collins MO, Shaw JG & Green LR (2025) . The FEBS Journal.
  • Jadi PK, Dave A, Issa R, Tabbasum K, Okurowska K, Samarth A, Urwin L, Green LR, Partridge LJ, MacNeil S , Garg P et al (2024) . The Ocular Surface, 32, 211-218.
  • Green LR, Issa R, Albaldi F, Urwin L, Thompson R, Khalid H, Turner CE, Ciani B, Partridge LJ & Monk PN (2023) . mBio, 14(4).
  • Cotton S, McHugh MP, Dewar R, Haas JG, Templeton K, Robson SC, Connor TR, Loman NJ, Golubchik T, Martinez Nunez RT , Bonsall D et al (2023) . Journal of Hospital Infection, 135, 28-36.
  • Dave N, Albiheyri RS, Wanford JJ, Green LR, Oldfield NJ, Turner DPJ, Martinez-Pomares L & Bayliss CD (2023) . Microbiology, 169(2).
  • Wilkinson SAJ, Richter A, Casey A, Osman H, Mirza JD, Stockton J, Quick J, Ratcliffe L, Sparks N, Cumley N , Poplawski R et al (2022) . Virus Evolution, 8(2).
  • Eales O, Page AJ, de Oliveira Martins L, Wang H, Bodinier B, Haw D, Jonnerby J, Atchison C, Robson SC, Connor TR , Loman NJ et al (2022) . BMC Infectious Diseases, 22(1).
  • Kemp SA, Collier DA, Datir RP, Ferreira IATM, Gayed S, Jahun A, Hosmillo M, Rees-Spear C, Mlcochova P, Lumb IU , Roberts DJ et al (2022) . Nature, 608(7922), E23-E23.
  • Collier DA, De Marco A, Ferreira IATM, Meng B, Datir RP, Walls AC, Kemp SA, Bassi J, Pinto D, Silacci-Fregni C , Bianchi S et al (2022) . Nature, 608(7922), E24-E24.
  • Smallman-Raynor MR & Cliff AD (2022) . Epidemiology and Infection, 150.
  • Parker MD, Stewart H, Shehata OM, Lindsey BB, Shah DR, Hsu S, Keeley AJ, Partridge DG, Leary S, Cope A , State A et al (2022) . Communications Biology, 5.
  • Vöhringer HS, Sanderson T, Sinnott M, De Maio N, Nguyen T, Goater R, Schwach F, Harrison I, Hellewell J, Ariani CV , Gonçalves S et al (2022) . Nature, 606(7915), E18-E18.
  • Ashford F, Best A, Dunn SJ, Ahmed Z, Siddiqui H, Melville J, Wilkinson S, Mirza J, Cumley N, Stockton J , Ferguson J et al (2022) . Journal of Clinical Microbiology, 60(4).
  • Wright DW, Harvey WT, Hughes J, Cox M, Peacock TP, Colquhoun R, Jackson B, Orton R, Nielsen M, Hsu NS , Harrison EM et al (2022) . Virus Evolution, 8(1), 1-14.
  • Aggarwal D, Page AJ, Schaefer U, Savva GM, Myers R, Volz E, Ellaby N, Platt S, Groves N, Gallagher E , Tumelty NM et al (2022) . Nature Communications, 13(1).
  • Green LR, Cole J, Parga EFD & Shaw JG (2022) . Advances in microbial physiology, 80, 35-83.
  • Lindsey BB, Villabona-Arenas CJ, Campbell F, Keeley AJ, Parker MD, Shah DR, Parsons H, Zhang P, Kakkar N, Gallis M , Foulkes BH et al (2022) . Nat Commun, 13(1), 1013.
  • Aggarwal D, Warne B, Jahun AS, Hamilton WL, Fieldman T, du Plessis L, Hill V, Blane B, Watkins E, Wright E , Hall G et al (2022) . Nature Communications, 13(1).
  • Lindsey BB, Villabona-Arenas CJ, Campbell F, Keeley AJ, Parker MD, Shah DR, Parsons H, Zhang P, Kakkar N, Gallis M , Foulkes BH et al (2022) . Nature Communications, 13(1).
  • Twohig KA, Nyberg T, Zaidi A, Thelwall S, Sinnathamby MA, Aliabadi S, Seaman SR, Harris RJ, Hope R, Lopez-Bernal J , Gallagher E et al (2022) . The Lancet Infectious Diseases, 22(1), 35-42.
  • Vöhringer HS, Sanderson T, Sinnott M, De Maio N, Nguyen T, Goater R, Schwach F, Harrison I, Hellewell J, Ariani CV , Gonçalves S et al (2021) . Nature, 600(7889), 506-511.
  • de Silva TI, Liu G, Lindsey BB, Dong D, Moore SC, Hsu NS, Shah D, Wellington D, Mentzer AJ, Angyal A , Brown R et al (2021) . iScience, 24(11).
  • Jackson B, Boni MF, Bull MJ, Colleran A, Colquhoun RM, Darby AC, Haldenby S, Hill V, Lucaci A, McCrone JT , Nicholls SM et al (2021) . Cell, 184(20), 5179-5188.e8.
  • Meng B, Kemp SA, Papa G, Datir R, Ferreira IATM, Marelli S, Harvey WT, Lytras S, Mohamed A, Gallo G , Thakur N et al (2021) . Cell Reports, 35(13), 109292-109292.
  • Partridge LJ, Urwin L, Nicklin MJH, James DC, Green LR & Monk PN (2021) . Cells, 10(6).
  • Volz E, Mishra S, Chand M, Barrett JC, Johnson R, Geidelberg L, Hinsley WR, Laydon DJ, Dabrera G, O’Toole Á , Amato R et al (2021) . Nature, 593(7858), 266-269.
  • Graham MS, Sudre CH, May A, Antonelli M, Murray B, Varsavsky T, Kläser K, Canas LS, Molteni E, Modat M , Drew DA et al (2021) . The Lancet Public Health, 6(5), e335-e345.
  • Parker MD, Lindsey BB, Leary S, Gaudieri S, Chopra A, Wyles M, Angyal A, Green LR, Parsons P, Tucker RM , Brown R et al (2021) . Genome Research, 31(4), 645-658.
  • Collier DA, De Marco A, Ferreira IATM, Meng B, Datir RP, Walls AC, Kemp SA, Bassi J, Pinto D, Silacci-Fregni C , Bianchi S et al (2021) . Nature, 593(7857), 136-141.
  • Kemp SA, Collier DA, Datir RP, Ferreira IATM, Gayed S, Jahun A, Hosmillo M, Rees-Spear C, Mlcochova P, Lumb IU , Roberts DJ et al (2021) . Nature, 592(7853), 277-282.
  • Thomson EC, Rosen LE, Shepherd JG, Spreafico R, da Silva Filipe A, Wojcechowskyj JA, Davis C, Piccoli L, Pascall DJ, Dillen J , Lytras S et al (2021) . Cell, 184(5), 1171-1187.e20.
  • Remmington A, Haywood S, Edgar J, Green LR, de Silva T & Turner CE (2021) . Microbial Genomics, 7(1).
  • Volz E, Hill V, McCrone JT, Price A, Jorgensen D, O’Toole Á, Southgate J, Johnson R, Jackson B, Nascimento FF , Rey SM et al (2021) . Cell, 184(1), 64-75.e11.
  • Urwin L, Okurowska K, Crowther G, Roy S, Garg P, Karunakaran E, MacNeil S, Partridge LJ, Green LR & Monk PN (2020) . Cells, 9(11).
  • Korber B, Fischer WM, Gnanakaran S, Yoon H, Theiler J, Abfalterer W, Hengartner N, Giorgi EE, Bhattacharya T, Foley B , Hastie KM et al (2020) . Cell, 182(4), 812-827.e19.
  • Alm E, Broberg EK, Connor T, Hodcroft EB, Komissarov AB, Maurer-Stroh S, Melidou A, Neher RA, O’Toole Á & Pereyaslov D (2020) . Eurosurveillance, 25(32).
  • The COVID-19 Genomics UK (COG-UK) consortium (2020) . The Lancet Microbe, 1(3), e99-e100.
  • Wanford JJ, Holmes JC, Bayliss CD & Green LR (2020) . Microbial Genomics, 6(5).
  • Green LR, Al-Rubaiawi AA, Al-Maeni MARM, Harrison OB, Blades M, Oldfield NJ, Turner DPJ, Maiden MCJ & Bayliss CD (2020) . mBio, 11(2).
  • Gray K, Green LR, Chaudhuri RR & Shaw JG (2019) . Microbiology Resource Announcements, 8(30).
  • Green LR, Dave N, Adewoye AB, Lucidarme J, Clark SA, Oldfield NJ, Turner DPJ, Borrow R & Bayliss CD (2019) . The Journal of Infectious Diseases, 220(7), 1109-1117.
  • Dave N, Green L, Lucidarme J, Oldfield N & Bayliss C (2019) . Access Microbiology, 1(1A).
  • Aidley J, Wanford JJ, Green LR, Sheppard SK & Bayliss CD (2018) . Microbial Genomics, 4(11).
  • Green LR, Lucidarme J, Dave N, Chan H, Clark S, Borrow R & Bayliss CD (2018) . Journal of Clinical Microbiology, 56(9).
  • Oldfield NJ, Green LR, Parkhill J, Bayliss CD & Turner DPJ (2018) . The Journal of Infectious Diseases, 217(4), 608-616.
  • Oldfield NJ, Cayrou C, AlJannat MAK, Al-Rubaiawi AAA, Green LR, Dada S, Steels OD, Stirrup C, Wanford J, Atwah BAY , Bayliss CD et al (2017) . Emerging Infectious Diseases, 23(6), 1009-1011.
  • Scully IL, Swanson K, Green L, Jansen KU & Anderson AS (2015) . Current Opinion in Microbiology, 27, 96-102.
  • Green LR, Monk PN, Partridge LJ, Morris P, Gorringe AR & Read RC (2011) . Infect Immun, 79(6), 2241-2249.
  • Pascall DJ, Vink E, Blacow R, Bulteel N, Campbell A, Campbell R, Clifford S, Davis C, da Silva Filipe A, El Sakka N , Fjodorova L et al () . PLOS ONE, 18(4), e0284187-e0284187.
  • Willett BJ, Grove J, MacLean OA, Wilkie C, De Lorenzo G, Furnon W, Cantoni D, Scott S, Logan N, Ashraf S , Manali M et al () . Nature Microbiology, 7(10), 1709-1709.
  • Nickbakhsh S, Hughes J, Christofidis N, Griffiths E, Shaaban S, Enright J, Smollett K, Nomikou K, Palmalux N, Tong L , Carmichael S et al () . Scientific Reports, 12(1).
  • Willett BJ, Grove J, MacLean OA, Wilkie C, De Lorenzo G, Furnon W, Cantoni D, Scott S, Logan N, Ashraf S , Manali M et al () . Nature Microbiology, 7(8), 1161-1179.
  • Kläser K, Molteni E, Graham M, Canas LS, Österdahl MF, Antonelli M, Chen L, Deng J, Murray B, Kerfoot E , Wolf J et al () . Scientific Reports, 12(1).
  • Holmes JC, Green LR, Oldfield NJ, Turner DPJ & Bayliss CD () . Frontiers in Genetics, 11.
  • Cayrou C, Akinduko AA, Mirkes EM, Lucidarme J, Clark SA, Green LR, Cooper HJ, Morrissey J, Borrow R & Bayliss CD () . PLOS ONE, 13(5), e0197186-e0197186.
  • Wanford JJ, Green LR, Aidley J & Bayliss CD () . PLOS ONE, 13(5), e0196675-e0196675.

Book chapters

  • Green LR, Haigh RD & Bayliss CD (2019) , Methods in Molecular Biology (pp. 83-92). Springer New York
  • Green LR, Eiden J, Hao L, Jones T, Perez J, McNeil LK, Jansen KU & Anderson AS (2016) , Methods in Molecular Biology (pp. 445-469). Springer New York

Preprints

  • Wolverson PA, Fernandes Parreira I, Collins MO, Shaw JG & Green LR (2024) , Cold Spring Harbor Laboratory.
  • Ghafari M, Hall M, Golubchik T, Ayoubkhani D, House T, MacIntyre-Cockett G, Fryer H, Thomson L, Nurtay A, Buck D , Green A et al (2023) , Cold Spring Harbor Laboratory.
  • Green LR, Issa R, Albaldi F, Urwin L, Khalid H, Turner CE, Ciani B, Partridge LJ & Monk PN (2023) , Cold Spring Harbor Laboratory.
  • Tsui JL-H, Lambert B, Bajaj S, McCrone JT, Inward RPD, Bosetti P, Hill V, Pena RE, Zarebski AE, Peacock TP , Liu L et al (2023) , Cold Spring Harbor Laboratory.
  • Fryer HR, Golubchik T, Hall M, Fraser C, Hinch R, Ferretti L, Thomson L, Nurtay A, Pellis L, MackIntyre-Cockett G , Trebes A et al (2022) , Cold Spring Harbor Laboratory.
  • Wilkinson SAJ, Richter A, Casey A, Osman H, Mirza JD, Stockton J, Quick J, Ratcliffe L, Sparks N, Cumley N , Poplawski R et al (2022) , Cold Spring Harbor Laboratory.
  • Smallman-Raynor MR & Cliff AD (2022) , Cold Spring Harbor Laboratory.
  • Lythgoe K, Golubchik T, Hall M, House T, Cahuantzi R, MacIntyre-Cockett G, Fryer H, Thomson L, Nurtay A, Ghafani M , Buck D et al (2022) , Cold Spring Harbor Laboratory.
  • Willett BJ, Grove J, MacLean OA, Wilkie C, Logan N, Lorenzo GD, Furnon W, Scott S, Manali M, Szemiel A , Ashraf S et al (2022) , Cold Spring Harbor Laboratory.
  • McCrone JT, Hill V, Bajaj S, Pena RE, Lambert BC, Inward R, Bhatt S, Volz E, Ruis C, Dellicour S , Baele G et al (2021) , Cold Spring Harbor Laboratory.
  • O’Toole Á, Hill V, Jackson B, Dewar R, Sahadeo N, Colquhoun R, Rooke S, McCrone JT, McHugh MP, Nicholls S , Poplawski R et al (2021) , Cold Spring Harbor Laboratory.
  • Parker MD, Lindsey BB, Shah DR, Hsu S, Keeley AJ, Partridge DG, Leary S, Cope A, State A, Johnson K , Ali N et al (2021) , Cold Spring Harbor Laboratory.
  • Graham MS, Sudre CH, May A, Antonelli M, Murray B, Varsavsky T, Kläser K, Canas LS, Molteni E, Modat M , Drew DA et al (2021) , Cold Spring Harbor Laboratory.
  • Collier DA, De Marco A, Ferreira IATM, Meng B, Datir R, Walls AC, Kemp S SA, Bassi J, Pinto D, Fregni CS , Bianchi S et al (2021) , Cold Spring Harbor Laboratory.
  • Golubchik T, Lythgoe KA, Hall M, Ferretti L, Fryer HR, MacIntyre-Cockett G, de Cesare M, Trebes A, Piazza P, Buck D , Todd JA et al (2021) , Cold Spring Harbor Laboratory.
  • Mashe T, Takawira FT, Martins LDO, Gudza-Mugabe M, Chirenda J, Munyanyi M, Chaibva BV, Tarupiwa A, Gumbo H, Juru A , Nyagupe C et al (2021) , Cold Spring Harbor Laboratory.
  • Volz E, Mishra S, Chand M, Barrett JC, Johnson R, Geidelberg L, Hinsley WR, Laydon DJ, Dabrera G, O’Toole Á , Amato R et al (2021) , Cold Spring Harbor Laboratory.
  • Davies NG, Abbott S, Barnard RC, Jarvis CI, Kucharski AJ, Munday JD, Pearson CAB, Russell TW, Tully DC, Washburne AD , Wenseleers T et al (2020) , Cold Spring Harbor Laboratory.
  • Thomson EC, Rosen LE, Shepherd JG, Spreafico R, da Silva Filipe A, Wojcechowskyj JA, Davis C, Piccoli L, Pascall DJ, Dillen J , Lytras S et al (2020) , Cold Spring Harbor Laboratory.
  • Nicholls SM, Poplawski R, Bull MJ, Underwood A, Chapman M, Abu-Dahab K, Taylor B, Jackson B, Rey S, Amato R , Livett R et al (2020) , Cold Spring Harbor Laboratory.
  • Partridge LJ, Urwin L, Nicklin MJH, James DC, Green LR & Monk PN (2020) , Cold Spring Harbor Laboratory.
Research group

We are proud to consist of a global collection of postdoctoral researchers, PhD students and Master's/project students:

PDRA

Dr Isabel Fernandes Parreira - Investigating 3D models of sex-specific N. gonorrhoeae infection

PhD students

Miss Paige Wolverson - 'The effect of novel anti-infectives across the meningococcal infective pathway and in a 3D blood-brain barrier spheroid model'

Miss Anisha Dhanjal - 'Improving diagnostics of antimicrobial resistant N. gonorrhoeae using long-read sequencing'

Miss Luisa Carvalho - 'Determining the importance of LOS phase variation during sex-specific N. gonorrhoeae infection'

Grants
  • The Humane Research Trust Research Fellowship.
  • THRT PhD Scholarship.
Opportunities

Postdoctoral funding

Enquiries are welcome from talented postdoctoral researchers interested in schemes such as the Newton Fellowships or Marie Curie Incoming fellowships at all times. Please contact Dr Luke Green for further information.

PhD projects

Enquiries from committed and talented prospective PhD students are welcome in all areas of our research portfolio. For further information contact Dr Luke Green for details. We welcome enquiries from funded overseas and home applicants.

Submit an enquiry