Professor David James

School of Chemical, Materials and Biological Engineering

Professor of Bioprocess Engineering

Research Centre Director Advanced Biomanufacturing Centre

Professor David James stands in the Engineering Heartspace
Profile picture of Professor David James stands in the Engineering Heartspace
d.c.james@sheffield.ac.uk
+44 114 222 7505
Room D62, Sir Robert Hadfield Building

Full contact details

Professor David James
School of Chemical, Materials and Biological Engineering
Room D62
Sir Robert Hadfield Building
Mappin Street
Sheffield
S1 3JD
Profile

I graduated in 1989 from King織s College London with a Ph.D. in Biochemistry, followed by postdoctoral research in Wye College (University of London), then the University of Kent; the latter a joint project with the Advanced Centre for Biochemical Engineering at University College London.

Following appointment to Lecturer, then Senior Lecturer at Kent, I moved to the University of Queensland in 2001 as an Associate Professor of Bioengineering, then Professorial Research Fellow in the Australian Institute of Bioengineering and Nanotechnology.

I was appointed to a Chair in Bioprocess Engineering at Sheffield in 2006 with a Royal Society Wolfson Research Merit Award, Research and Training in Biopharmaceutical Bioprocessing for UK Bioindustry.

Research interests
  • Production of high-value therapeutic recombinant protein biopharmaceuticals
  • Systems Biotechnology for Rational Bioprocess Engineering
  • Cell Factory and Gene Vector Engineering
  • Process Analytical Technology
Publications

Books

  • McLeod J & James DC (2011) .
  • Davies SL & James DC (2009) . Springer Netherlands.

Journal articles

  • Draper BJ, Dunning MJ & James DC (2025) . F1000Research, 14.
  • Draper BJ, Dunning MJ & James DC (2025) . F1000Research, 14.
  • Evans C (2024) Glycoprofile Comparison of the SARS-CoV-2 Spike Proteins Expressed in CHO and HEK Cell Lines. Marine Biotechnology.
  • Johari YB, Pohle TH, Whitehead J, Scarrott JM, Liu P, Mayer A & James DC (2024) . Biotechnology Journal, 19(6).
  • Syddall KL, FernandezMartell A, Cartwright JF, Alexandru-Crivac CN, Hodgson A, Racher AJ, Young RJ & James DC (2024) . Metabolic Engineering, 81, 53-69.
  • ONeill P, Mistry RK, Brown AJ & James DC (2023) . ACS Synthetic Biology, 12(8), 2339-2352.
  • Sou SN, Harris CL, Williams R, Kozub D, Zurlo F, Patel YD, Kallamvalli Illam Sankaran P, Daramola O, Brown A, James DC , Hatton D et al (2023) . Biotechnology Progress, 39(5).
  • Lambiase G, Klottrup-Rees K, Lovelady C, Ali S, Shepherd S, Muroni M, Lindo V, James DC & Dickman MJ (2023) . Journal of Chromatography A, 1691.
  • Scarrott JM, Johari YB, Pohle TH, Liu P, Mayer A & James DC (2023) . Biotechnology Journal, 18(3).
  • Colton H, Hodgson D, Hornsby H, Brown R, Mckenzie J, Bradley KL, James C, Lindsey BB, Birch S, Marsh L , Wood S et al (2022) . Wellcome Open Research, 6.
  • Johari YB, Scarrott JM, Pohle TH, Liu P, Mayer A, Brown AJ & James DC (2022) . Biotechnology Journal, 17(8).
  • Johnson AO, Fowler SB, Webster CI, Brown AJ & James DC (2022) . ACS Synthetic Biology, 11(4), 1613-1626.
  • Colton H, Hodgson D, Hornsby H, Brown R, Mckenzie J, Bradley KL, James C, Lindsey BB, Birch S, Marsh L , Wood S et al (2022) . Wellcome Open Research, 6.
  • Lambiase G, Inman SE, Muroni M, Lindo V, Dickman MJ & James DC (2022) . Journal of Chromatography A, 1670.
  • Colton H, Hodgson D, Hornsby H, Brown R, Mckenzie J, Bradley KL, James C, Lindsey BB, Birch S, Marsh L , Wood S et al (2021) . Wellcome Open Research, 6.
  • Partridge LJ, Urwin L, Nicklin MJH, James DC, Green LR & Monk PN (2021) . Cells, 10(6).
  • Patel YD, Brown AJ, Zhu J, Rosignoli G, Gibson SJ, Hatton D & James DC (2021) . ACS Synthetic Biology, 10(5), 1155-1165.
  • Johari YB, Mercer AC, Liu Y, Brown AJ & James DC (2021) . Biotechnology and Bioengineering, 118(5), 2001-2015.
  • Johari YB, Jaff矇 SRP, Scarrott JM, Johnson AO, Mozzanino T, Pohle TH, Maisuria S, BhayatCammack A, Lambiase G, Brown AJ , Tee KL et al (2021) . Biotechnology and Bioengineering, 118(2), 1013-1021.
  • ToronjoUrquiza L, AcostaMartin AE, James DC, Nagy T & Falconer RJ (2020) . Biotechnology Progress, 36(4).
  • ToronjoUrquiza L, AcostaMartin AE, James DC, Nagy T & Falconer RJ (2020) . Biotechnology Progress, 36(3).
  • Dobson PD, Coss KP, Doherty C, Clifford J, Thompson B & James DC (2020) . Biotechnology and Bioengineering, 117(7), 2295-2299.
  • Cartwright JF, Arnall CL, Patel YD, Barber NOW, Lovelady CS, Rosignoli G, Harris CL, Dunn S, Field RP, Dean G , Daramola O et al (2020) . Journal of Biotechnology, 312, 11-22.
  • Cole J, Hanson EJ, James DC, Dockrell DH & Dickman MJ (2019) . Rapid Communications in Mass Spectrometry, 33(10), 897-906.
  • Johari YB, Brown AJ, Alves CS, Zhou Y, Wright CM, Estes SD, Kshirsagar R & James DC (2019) . Journal of Biotechnology, 294, 1-13.
  • Brown AJ, Gibson SJ, Hatton D, Arnall CL & James DC (2019) . Biotechnology and Bioengineering, 116(2), 375-387.
  • Geoghegan D, Arnall C, Hatton D, Noble-Longster J, Sellick C, Senussi T & James DC (2018) . Biotechnology and Bioengineering, 115(12), 2908-2929.
  • Cartwright JF, Anderson K, Longworth J, Lobb P & James DC (2018) . Biotechnology and Bioengineering, 115(6), 1485-1498.
  • Fernandez-Martell A, Johari YB & James DC (2018) . Biotechnology and Bioengineering, 115(3), 645-660.
  • Brown AJ, Gibson S, Hatton D & James DC (2018) . Biotechnology Journal, 13(1).
  • Brown AJ, Gibson SJ, Hatton D & James DC (2017) . Nucleic Acids Research, 45(18), 10906-10919.
  • Thompson B, Clifford J, Jenns M, Smith A, Field R, Nayyar K & James DC (2017) . Analytical Biochemistry, 534, 49-55.
  • Brown AJ & James DC (2017) , 131-145.
  • Brown AJ & James DC (2016) . Biotechnology Advances, 34(5), 492-503.
  • Walther C, Whitfield R & James DC (2015) . Applied Biochemistry and Biotechnology, 178(7), 1286-1302.
  • Johari YB, Estes SD, Alves CS, Sinacore MS & James DC (2015) . Biotechnology and Bioengineering, 112(12), 2527-2542.
  • Brown AJ, Sweeney B, Mainwaring DO & James DC (2015) . Biotechnology Journal, 10(7), 1019-1028.
  • Brown AJ, Sweeney B, Mainwaring DO & James DC (2014) . Biotechnol Bioeng, 111(8), 1638-1647.
  • Mozley OL, Thompson BC, Fernandez-Martell A & James DC (2014) . Biotechnol Prog, 30(5), 1161-1170.
  • Pybus LP, James DC, Dean G, Slidel T, Hardman C, Smith A, Daramola O & Field R (2014) . Biotechnol Prog, 30(1), 188-197.
  • Pybus LP, Dean G, West NR, Smith A, Daramola O, Field R, Wilkinson SJ & James DC (2014) . Biotechnol Bioeng, 111(2), 372-385.
  • Brown AJ, Mainwaring DO, Sweeney B & James DC (2013) . Anal Biochem, 443(2), 205-210.
  • Grainger RK & James DC (2013) . Biotechnol Bioeng, 110(11), 2970-2983.
  • Davies SL, Lovelady CS, Grainger RK, Racher AJ, Young RJ & James DC (2013) . Biotechnol Bioeng, 110(1), 260-274.
  • Guesdon F, Kaabi Y, Riley AH, Wilkinson IR, Gray C, James DC, Artymiuk PJ, Sayers JR & Ross RJ (2012) . Biosci Rep, 32(6), 653-660.
  • Thompson BC, Segarra CRJ, Mozley OL, Daramola O, Field R, Levison PR & James DC (2012) . Biotechnol Prog, 28(1), 179-187.
  • Davies SL, O'Callaghan PM, McLeod J, Pybus LP, Sung YH, Rance J, Wilkinson SJ, Racher AJ, Young RJ & James DC (2011) . Biotechnol Prog, 27(6), 1689-1699.
  • Kim M, O'Callaghan PM, Droms KA & James DC (2011) . Biotechnol Bioeng, 108(10), 2434-2446.
  • McLeod J, O'Callaghan PM, Pybus LP, Wilkinson SJ, Root T, Racher AJ & James DC (2011) . Biotechnol Bioeng, 108(9), 2193-2204.
  • O'Callaghan PM, McLeod J, Pybus LP, Lovelady CS, Wilkinson SJ, Racher AJ, Porter A & James DC (2010) . Biotechnol Bioeng, 106(6), 938-951.
  • O'Callaghan PM & James DC (2008) . Brief Funct Genomic Proteomic, 7(2), 95-110.
  • Underhill MF, Smales CM, Naylor LH, Birch JR & James DC (2007) . Biotechnology Progress, 23(2), 435-443.
  • Stansfield SH, Allen EE, Dinnis DM, Racher AJ, Birch JR & James DC (2007) Dynamic analysis of GS-NS0 cells producing a recombinant monoclonal antibody during fed-batch culture. BIOTECHNOL BIOENG, 97(2), 410-424.
  • Galbraith DJ, Tait AS, Racher AJ, Birch JR & James DC (2006) . Biotechnology Progress, 22(3), 753-762.
  • Ahmad N, Zhang J, Brown PJ, James DC, Birch JR, Racher AJ & Smales CM (2006) . Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 1764(7), 1179-1187.
  • Dinnis DM, Stansfield SH, Schlatter S, Smales CM, Alete D, Birch JR, Racher AJ, Marshall CT, Nielsen LK & James DC (2006) . Biotechnology and Bioengineering, 94(5), 830-841.
  • Weeks ME, Nebe von Caron G, James DC, Smales CM & Robinson GK (2006) . Journal of Microbiological Methods, 66(1), 43-55.
  • Hodoniczky J, Zheng YZ & James DC (2005) . Biotechnology Progress, 21(6), 1644-1652.
  • Alete DE, Racher AJ, Birch JR, Stansfield SH, James DC & Smales CM (2005) . PROTEOMICS, 5(18), 4689-4704.
  • Bailey MJ, Hooker AD, Adams CS, Zhang S & James DC (2005) . Journal of Chromatography B, 826(1-2), 177-187.
  • Dinnis DM & James DC (2005) . Biotechnology and Bioengineering, 91(2), 180-189.
  • Tait AS, Brown CJ, Galbraith DJ, Hines MJ, Hoare M, Birch JR & James DC (2004) . Biotechnology and Bioengineering, 88(6), 707-721.
  • Smales CM, Dinnis DM, Stansfield SH, Alete D, Sage EA, Birch JR, Racher AJ, Marshall CT & James DC (2004) . Biotechnology and Bioengineering, 88(4), 474-488.
  • Weeks ME, James DC, Robinson GK & Smales CM (2004) . PROTEOMICS, 4(1), 123-135.
  • Underhill MF, Coley C, Birch JR, Findlay A, Kallmeier R, Proud CG & James DC (2003) . Biotechnology Progress, 19(1), 121-129.
  • Mark Smales C, Birch JR, Racher AJ, Marshall CT & James DC (2003) . Biochemical and Biophysical Research Communications, 306(4), 1050-1055.
  • Harris L (2003) . Molecular Immunology, 40(6), 381-389.
  • Baker KN, Rendall MH, Patel A, Boyd P, Hoare M, Freedman RB & James DC (2002) . Trends in Biotechnology, 20(4), 149-156.
  • Smales CM, Pepper DS & James DC (2002) . Biotechnology and Bioengineering, 77(1), 37-48.
  • Smales CM, Pepper DS & James DC (2001) . Biotechnology Progress, 17(5), 974-978.
  • Hills AE, Patel A, Boyd P & James DC (2001) . Biotechnology and Bioengineering, 75(2), 239-251.
  • Baker KN, Rendall MH, Hills AE, Hoare M, Freedman RB & James DC (2001) . Biotechnology and Bioengineering, 73(3), 188-202.
  • Smales CM, Pepper DS & James DC (2001) . Rapid Communications in Mass Spectrometry, 15(5), 351-356.
  • Smales CM, Pepper DS & James DC (2000) . Biotechnology and Applied Biochemistry, 32(2), 109-119.
  • Baker KN, Cook A, Roberts G, Gerardy-Schahn R, Baines A & James DC (2000) . Biochemical Society Transactions, 28(5), A259-A259.
  • Smales CM, Pepper DS & James DC (2000) . Biotechnology and Bioengineering, 67(2), 177-188.
  • Hills AE, Patel AK, Boyd PN & James DC (2000) Metabolic control of recombinant monoclonal antibody N-glycosylation in GS-NS0 cells. GLYCOBIOLOGY, 10(10), 1108-1108.
  • Hooker AD, Green NH, Baines AJ, Bull AT, Jenkins N, Strange PG & James DC (1999) . Biotechnology and Bioengineering, 63(5), 559-572.
  • Lagutin OV, Dobrovolsky VN, Vinogradova TV, Kyndiakov BN, Khodarovich YM, Jenkins N, James D, Markham N & Larionov OA (1999) . Journal of Interferon & Cytokine Research, 19(2), 137-144.
  • Lamb EJ, Cleave SJ, Smales M & James DC (1999) Characterisation of glycated albumin using electrospray ionisation mass spectrometry. Clinical Chemistry and Laboratory Medicine, 37(SPEC. SUPPL.), S520-S520.
  • Goldman MH, James DC, Rendall M, Ison AP, Hoare M & Bull AT (1998) . Biotechnology and Bioengineering, 60(5), 596-607.
  • HOOKER A & JAMES D (1998) . Journal of Interferon & Cytokine Research, 18(5), 287-295.
  • Baker K, Ison A, Freedman R, Jones W & James D (1997) Real-time monitoring of recombinant protein concentration in animal cell cultures using an optical biosensor. Genetic Engineer and Biotechnologist, 17(2-3), 69-74.
  • Goldman MH, James DC, Ison AP & Bull AT (1997) . Cytotechnology, 23(1-3), 103-111.
  • Freedman RB, Jenkins N, James DC, Goldman MH, Hoare M, Oliver RWA & Green BN (1996) . Protein Science, 5(2), 331-340.
  • Jenkins N, Parekh RB & James DC (1996) . Nature Biotechnology, 14(8), 975-981.
  • James DC (1996) . Cytotechnology, 22(1-3), 17-24.
  • Hooker AD, Goldman MH, Markham NH, James DC, Ison AP, Bull AT, Strange PG, Salmon I, Baines AJ & Jenkins N (1995) . Biotechnology and Bioengineering, 48(6), 639-648.
  • James DC, Freedman RB, Hoare M, Ogonah OW, Rooney BC, Larionov OA, Dobrovolsky VN, Lagutin OV & Jenkins N (1995) . Nature Biotechnology, 13(6), 592-596.
  • James DC, Freedman RB, Hoare M & Jenkins N (1994) . Analytical Biochemistry, 222(2), 315-322.
  • BESTWICK LA, GRONNING LM, JAMES DC, BONES A & ROSSITER JT (1993) PURIFICATION AND CHARACTERIZATION OF A NITRILASE FROM BRASSICA-NAPUS. PHYSIOLOGIA PLANTARUM, 89(4), 811-816.
  • JAMES DC & ROSSITER JT (1991) DEVELOPMENT AND CHARACTERISTICS OF MYROSINASE IN BRASSICA-NAPUS DURING EARLY SEEDLING GROWTH. PHYSIOLOGIA PLANTARUM, 82(2), 163-170.
  • Rossiter JT & James DC (1990) BIOSYNTHESIS OF R-2 HYDROXYBUT-3-ENYLGLUCOSINOLATE PROGOITRIN FROM 3 4 TRITIATED BUT-3-ENYLGLUCOSINOLATE IN BRASSICA-NAPUS. Journal of the Chemical Society Perkin Transactions I(7), 1909-1914.
  • Rossiter JT & James DC (1990) . Journal of the Chemical Society, Perkin Transactions 1(7), 1909-1909.
  • Rossiter JT, James DC & Atkins N (1990) . Phytochemistry, 29(8), 2509-2512.
  • Urquiza LT, James DC, Nagy T & Falconer RJ () . Antioxidants, 8(6).
  • Schlatter S, Stansfield SH, Dinnis DM, Racher AJ, Birch JR & James DC () . Biotechnology Progress, 21(1), 122-133.
  • Underhill MF, Marchant RJ, Carden MJ, James DC & Smales CM () . Molecular Biotechnology, 34(2), 141-150.
  • Hooker AD & James DC () . Molecular Biotechnology, 14(3), 241-249.
  • Hooker AD & James DC () , 277-288.

Book chapters

  • McLeod J & James DC (2019) , Comprehensive Biotechnology (pp. 405-412).
  • Brown AJ, Kalsi D, FernandezMartell A, Cartwright J, Barber NOW, Patel YD, Turner R, Bryant CL, Johari YB & James DC (2017) (pp. 423-467). Wiley
  • (2017) In Vaughan T, Osbourn J & Jallal B (Ed.) Wiley
  • McLeod J & James DC (2011) , Comprehensive Biotechnology (pp. 419-426). Elsevier
  • McLeod J & James DC (2011) , Comprehensive Biotechnology (pp. 405-412). Elsevier
  • Hooker AD & James DC (2009) , Springer Protocols Handbooks (pp. 1335-1346). Humana Press
  • Emerton V, Davis P, Smales CM & James DC (2002) , Food Allergy and Intolerance (pp. 150-161). The Royal Society of Chemistry
  • James DC & Baker KN (1999) Wiley
  • Hooker AD, Goldman MH, Green NH, James DC, Bull AT, Strange PG, Baines AJ & Jenkins N (1997) , Animal Cell Technology: Basic & Applied Aspects (pp. 315-321). Springer Netherlands
  • Smales CM, Marchant RJ & Underhill MF () , Therapeutic Proteins (pp. 375-380). Humana Press
  • Howard MJ () , Therapeutic Proteins (pp. 461-470). Humana Press

Conference proceedings

  • Scarrott JM, Johari YB, Pohle TH, Liu P, Mayer A & James DC (2022) Small molecule chemical additives for enhanced recombinant AAV production in HEK293 cells. HUMAN GENE THERAPY, Vol. 33(23-24) (pp A105-A105)
  • Ahmad N, Zhang J, Brown PJ, James DC, Birch JR, Racher AJ, Smales CM, Atan KAM & Krishnarajah IS (2008) . AIP Conference Proceedings, Vol. 971 (pp 60-64)
  • Davis PJ, Smales CM & James DC (2001) . Allergy, Vol. 56(s67) (pp 56-60)
  • Hills AE, Patel AK, Boyd PN & James DC (1999) Control of therapeutic monoclonal antibody glycosylation. ANIMAL CELL TECHNOLOGY: PRODUCTS FROM CELLS, CELLS AS PRODUCTS (pp 255-257)
  • Hooker AD, Goldman MH, Green NH, James DC, Bull AT, Strange PG, Baines AJ & Jenkin N (1997) Resolution glycoform analysis of recombinant human interferon-gamma during batch cultures of Chinese hamster ovary cells. ANIMAL CELL TECHNOLOGY: BASIC & APPLIED ASPECTS, VOL 8 (pp 315-321)
  • Green NH, Hooker AD, James DC, Baines AJ, Strange PG, Jenkins N & Bull AT (1997) (pp 339-345)
  • Hooker AD, Green NH, James DC, Strange PG, Baines AJ, Bull AT & Jenkins N (1997) (pp 277-282)
  • GOLDMAN MH, BULL AT, ISON AP, JAMES DC & JENKINS N (1995) A COMPARISON OF 2 ELECTROPHORETIC METHODS FOR ANALYSING RECOMBINANT PROTEIN GLYCOFORMS. 1995 ICHEME RESEARCH EVENT - FIRST EUROPEAN CONFERENCE FOR YOUNG RESEARCHERS IN CHEMICAL ENGINEERING, VOLS 1 AND 2 (pp 896-898)
  • Alete DE, Racher AJ, Birch JR, James DC & Smales CM () (pp 71-74)
  • Tait AS, Hoare M, Birch J, Galbraith DJ, Hines M, Brown CJ & James DC () (pp 655-657)
  • Baker KN, Johnson ID, Roberts G, Cook A, Baines A, Gerardy-Schahn R & James DC () (pp 263-265)

Patents

  • Brown AJ, James DC, Gibson SJ & Hatton D (2018) In silico design of mammalian promoters with user-defined functionality. WO 2018/193072 A1 Appl. 25 Oct 2018.
  • James DC & Brown AJ (2015) Synthetic promoters for CHO cells, methods of producing synthetic promoters using transcription factor binding site modules. WO2015079053-A2; WO2015079053-A3 Appl. 08 Oct 2015.
  • James DC & Brown AJ (2014) Regulating recombinant gene expression in vitro comprises providing a host cell encoding recombinant genes for expression, and contacting the cell with an exonuclease resistant block decoy. WO2014202640-A1 Appl. 24 Dec 2014.
  • Clifford J, James D & Thompson B () Predicting relative production titer of a panel of clonal producer cells in fed batch culture by simultaneously incubating each producer cell with a chemical cell stressor and determining the production titer response of each cell. EP2902493-A1; WO2015113704-A1 Appl. 01 Jan 1970.
  • Davies SL, James D & Thompson B () Method for determining e.g. cell identity of mammalian producer of user, involves comparing query cell-specific growth response fingerprint with reference cell-specific growth response fingerprints corresponding to cells. WO2014207166-A1 Appl. 01 Jan 1970.

Theses

  • Toronjo Urquiza L (2020) Screening and development of natural compounds as tools to improve IgG production and quality in recombinant CHO cell culture.

Preprints

  • Alexandru-Crivac CN, Cartwright JF, Taylor R, Sweeney BM, Feary M, Chathoth KT, Fabian DK, Allsopp H, Brown AJ & James DC (2024) , Cold Spring Harbor Laboratory.
  • ONeill P, Mistry RK, Brown AJ & James DC (2023) , Cold Spring Harbor Laboratory.
  • Scarrott J, Johari YB, Pohle T, Liu P, Mayer A & James D (2022) , Wiley.
  • Johari YB, Jaff矇 SRP, Scarrott JM, Johnson AO, Mozzanino T, Pohle TH, Maisuria S, Bhayat-Cammack A, Brown AJ, Tee KL , Jackson PJ et al (2020) , Cold Spring Harbor Laboratory.
  • Johari Y, Jaffe S, Scarrott J, Johnson A, Mozzanino TO, Pohle T, Maisuria S, Cammack AB, Brown A, Tee KL , Jackson P et al (2020) , Wiley.
  • Tee KL, Jackson PJ, Scarrott JM, Jaffe SRP, Johnson AO, Johari Y, Pohle TH, Mozzanino T, Price J, Grinham J , Brown A et al (2020) , Cold Spring Harbor Laboratory.
  • Partridge LJ, Urwin L, Nicklin MJH, James DC, Green LR & Monk PN (2020) , Cold Spring Harbor Laboratory.
  • Gonz獺lez J, Longworth J, James DC & Lawrence ND (2015) , arXiv.
  • Hodgson D, Colton H, Hornsby H, Brown R, Mckenzie J, Bradley KL, James C, Lindsey BB, Birch S, Marsh L , Wood S et al () .
Teaching activities

CPE412 Molecular Biotechnology