Professor Steve Winder

School of Biosciences

Emeritus Professor

Director of Recruitment and International Relations

s.winder@sheffield.ac.uk
B206, Firth Court

Full contact details

Professor Steve Winder
School of Biosciences
B206
Firth Court
Western Bank
Sheffield
S10 2TN
Profile

Brief career history

  • 2005-Present: Professor of Molecular Cell Biology, School of Biosciences, University of Sheffield
  • 2003-2005: Reader, Department of Biomedical Science, University of Sheffield
  • 1999-2003: Lecturer (99-01) Reader (02-03), University of Glasgow
  • 1995-1999: Wellcome Trust Fellow, ICMB, University of Edinburgh
  • 1992-1995: Staff Scientist, Laboratory of Molecular Biology, Cambridge - Advisor, Jake Kendrick-Jones
  • 1988-1992: Postdoctoral Fellow, Biochemistry, University of Calgary, Advisor - Mike Walsh.
  • 1984-1988: PhD, University of Reading, Supervisor - Isabel Forsyth
Research interests

Regulation of dystroglycan function in muscular dystrophy and cancer

The laminin binding protein dystroglycan plays multiple roles in cell adhesion, signalling and membrane cytoskeleton stability. Perturbation of dystroglycan function underlies several muscular dystrophies and is also a secondary consequence of adenocarcinoma progression.

Changes to the post-translational modification of dystroglycan are crucial in directing the associations, cellular localisation and ultimately degradation of dystroglycan.

Our aim is to elucidate the mechanisms and consequences of these post-translational modifications in order to better understand dystroglycan function and to identify potential therapeutic targets for the treatment of muscular dystrophy or cancer.

We employ in vitro, in/ex vivo fish and mouse genetic models with clinically relevant archival tissue samples or immortalised cell lines. Dystroglycan function is dissected through the use of molecular cell biology approaches, and potential therapeutic targets are assessed in vitro and in vivo.

Recently we have developed a novel therapeutic approach for the treatment of Duchenne muscular dystrophy using inhibitors of tyrosine phosphorylation and proteasomal degradation. Through the use of zebrafish screening and phenotypic analysis in mdx mice and human DMD myoblasts we are in the process of validating the potential for repurposed drugs as a precursor to initiating clinical trials.

Physiological analysis is carried out I collaboration with Nic Wells at the RVC London.

In vitro models of prostate cancer have revealed a role for the post-translational proteolytic processing and nuclear targeting of dystroglycan.

Current efforts are centred around characterising a role as part of the LINC complex in the inner nuclear membrane. These studies form part of an ongoing collaboration with Bulmaro Cisneros, CINVESTAV Mexico City.

Funding

  • Action Duchenne - Repurposed Cancer Therapeutics as Treatments for DMD
  • Duchenne UK - Are soy products effective in DMD?
  • White Rose - The Dystroglycan LINC
  • Action on Hearing Loss - Dystroglycan function in hearing and hearing loss
Publications

Show: Featured publications All publications

Journal articles

  • Lipscomb L, Piggott RW, Emmerson T & Winder SJ (2016) . Human Molecular Genetics, 25(2), 266-274.
  • Martínez-Vieyra IA, Vásquez-Limeta A, González-Ramírez R, Morales-Lázaro SL, Mondragón M, Mondragón R, Ortega A, Winder SJ & Cisneros B (2013) . Biochimica et Biophysica Acta - Molecular Cell Research, 1833(3), 698-711.
  • Mathew G, Mitchell A, Down JM, Jacobs LA, Hamdy FC, Eaton C, Rosario DJ, Cross SS & Winder SJ (2013) . Sci Rep, 3, 2792.
  • Miller G, Moore CJ, Terry R, La riviere T, Mitchell A, Piggott R, Dear TN, Wells DJ & Winder SJ (2012) . Human Molecular Genetics, 21(20), 4508-4520.
  • García‐Aguirre I, Alamillo‐Iniesta A, Rodríguez‐Pérez R, Vélez‐Aguilera G, Amaro‐Encarnación E, Jiménez‐Gutiérrez E, Vásquez‐Limeta A, Samuel Laredo‐Cisneros M, Morales‐Lázaro SL, Tiburcio‐Félix R , Ortega A et al () . Aging Cell.

All publications

Journal articles

  • Gómez-Monsivais WL, Monterrubio-Ledezma F, Huerta-Cantillo J, Mondragon-Gonzalez R, Alamillo-Iniesta A, García-Aguirre I, Azuara-Medina PM, Arguello-García R, Rivera-Monroy JE, Holaska JM , Hernández-Méndez JME et al (2020) . International Journal of Molecular Sciences, 21(17).
  • Mias-Lucquin D, Dos Santos Morais R, Chéron A, Lagarrigue M, Winder SJ, Chenuel T, Pérez J, Appavou M-S, Martel A, Alviset G , Le Rumeur E et al (2020) . Journal of Structural Biology, 209(1).
  • Azuara-Medina P, Sandoval-Duarte A, Morales-Lázaro S, Modragón-González R, Vélez-Aguilera G, Gómez-López J, Jiménez-Gutiérrez G, Tiburcio-Félix R, Martínez-Vieyra I, Sánchez R , Längst G et al (2019) . Cell Death and Disease, 10.
  • Gracida-Jiménez V, Mondragón-González R, Vélez-Aguilera G, Vásquez-Limeta A, Laredo-Cisneros MS, de Dios Gómez-López J, Vaca L, Gourlay SC, Jacobs LA, Winder SJ & Cisneros B (2018) . Scientific Reports, 8.
  • Vélez-Aguilera G, de Dios Gómez-López J, Jiménez-Gutiérrez GE, Vásquez-Limeta A, Laredo-Cisneros MS, Gómez P, Winder SJ & Cisneros B (2018) . Biochimica et Biophysica Acta - Molecular Cell Research, 1865(2), 406-420.
  • Gracida-Jiménez V, Mondragón-González R, Vélez-Aguilera G, Vásquez-Limeta A, Laredo-Cisneros MS, Gómez-López JDD, Vaca L, Gourlay SC, Jacobs LA, Winder SJ & Cisneros B (2017) . Scientific Reports, 7(1).
  • Alonso-Rangel L, Benítez-Guerrero T, Martínez-Vieyra I, Cisneros B, Martínez-Tovar A, Winder SJ & Cerecedo D (2017) . Life Sciences, 182, 1-9.
  • Brown SC & Winder SJ (2017) . Neuromuscular Disorders, 27(4).
  • Leocadio D, Mitchell A & Winder SJ (2016) . Journal of Cellular Biochemistry, 117(9), 2149-2157.
  • Hoffman E & Winder SJ (2016) . PLoS Currents.
  • Lipscomb L, Piggott RW, Emmerson T & Winder SJ (2016) . Human Molecular Genetics, 25(2), 266-274.
  • Escárcega-Tame MA, Martínez-Vieyra I, Alonso-Rangel L, Cisneros B, Winder SJ & Cerecedo D (2015) . PLOS ONE, 10(12), e0144078-e0144078.
  • Tetlow DJ, Winder SJ & Aïssa C (2015) . Chem. Commun., 52(4), 807-810.
  • Leiton CV, Aranmolate A, Eyermann C, Menezes MJ, Escobar-Hoyos LF, Husain S, Winder SJ & Colognato H (2015) . Journal of Neurochemistry, 135(3), 522-538.
  • Cerecedo D, Martínez-Vieyra I, Maldonado-García D, Hernández-González E & Winder SJ (2015) . Journal of Cellular Biochemistry, 116(11), 2528-2540.
  • Tame ME, Martinez-Vieyra I, Cisneros B, Winder SJ & Cerecedo D (2014) Dystroglycan depletion inhibits the functions of differentiated Kasumi-1 cells.. MOLECULAR BIOLOGY OF THE CELL, 25.
  • Martínez-Zárate AD, Martínez-Vieyra I, Alonso-Rangel L, Cisneros B, Winder SJ & Cerecedo D (2014) . Biochem Biophys Res Commun, 448(3), 274-280.
  • Singh SM, Bandi S, Winder SJ & Mallela KMG (2014) . Biochemistry, 53(11), 1801-1809.
  • Martínez-Vieyra IA, Vásquez-Limeta A, González-Ramírez R, Morales-Lázaro SL, Mondragón M, Mondragón R, Ortega A, Winder SJ & Cisneros B (2013) . Biochimica et Biophysica Acta - Molecular Cell Research, 1833(3), 698-711.
  • Mathew G, Mitchell A, Down JM, Jacobs LA, Hamdy FC, Eaton C, Rosario DJ, Cross SS & Winder SJ (2013) . Sci Rep, 3, 2792.
  • Sarkis J, Víe V, Winder SJ, Renault A, Le Rumeur E & Hubert JF (2013) . FASEB Journal, 27(1), 359-367.
  • Mitchell A, Mathew G, Jiang T, Hamdy FC, Cross SS, Eaton C & Winder SJ (2013) . Prostate, 73(4), 398-408.
  • Moore CJ & Winder SJ (2012) . Neuromuscul Disord, 22(11), 959-965.
  • Thompson O, Moghraby JS, Ayscough KR & Winder SJ (2012) . BMC Cell Biol, 13, 1.
  • Miller G, Moore CJ, Terry R, La riviere T, Mitchell A, Piggott R, Dear TN, Wells DJ & Winder SJ (2012) . Human Molecular Genetics, 21(20), 4508-4520.
  • Brown SC & Winder SJ (2012) . Neuromuscular Disorders, 22(7), 659-668.
  • Le Rumeur E, Hubert JF & Winder SJ (2012) . FEBS Letters, 586(17), 2717-2722.
  • Le Rumeur E, Hubert J-F & Winder SJ (2012) A new twist to coiled coil. FEBS Letters.
  • Broderick MJF, Bobkov A & Winder SJ (2012) . FEBS Open Bio, 2, 6-11.
  • Singh SM, Molas JF, Kongari N, Bandi S, Mallela KMG, Armstrong GS & Winder SJ (2012) . Proteins: Structure, Function and Bioformatics.
  • Brown SC & Winder SJ (2012) Dystroglycan and dystroglycanopathies: Report of the 187th ENMC Workshop 11-13 November 2011, Naarden, The Netherlands. Neuromuscular Disorders.
  • Parberry-Clark C, Bury JP, Cross SS & Winder SJ (2011) . Histopathology, 59(2), 180-187.
  • Winder SJ, Lipscomb L, Angela Parkin C & Juusola M (2011) . PLoS Curr, 3, RRN1286.
  • Le Rumeur E, Winder SJ & Hubert JF (2010) . BBA-PROTEINS PROTEOM, 1804(9), 1713-1722.
  • Moore CJ & Winder SJ (2010) . Cell Commun Signal, 8, 3.
  • Thompson O, Moore CJ, Hussain S-A, Kleino I, Peckham M, Hohenester E, Ayscough KR, Saksela K & Winder SJ (2010) . J Cell Sci, 123(Pt 1), 118-127.
  • Robertson AS, Allwood EG, Smith APC, Gardiner FC, Costa R, Winder SJ & Ayscough KR (2009) . Mol Biol Cell, 20(6), 1618-1628.
  • Cross SS, Lippitt J, Mitchell A, Hollingsbury F, Balasubramanian SP, Reed MWR, Eaton C, Catto JW, Hamdy F & Winder SJ (2008) . Histopathology, 53(5), 561-566.
  • Gheorghe DM, Aghamohammadzadeh S, Smaczynska-de Rooij II, Allwood EG, Winder SJ & Ayscough KR (2008) . J Biol Chem, 283(22), 15037-15046.
  • Bryant RJ, Winder SJ, Cross SS, Hamdy FC & Cunliffe VT (2008) . Prostate, 68(3), 255-263.
  • Thompson O, Kleino I, Crimaldi L, Gimona M, Saksela K & Winder SJ (2008) . PLoS One, 3(11), e3638.
  • Higginson JR, Thompson O & Winder SJ (2008) . Int J Biochem Cell Biol, 40(5), 892-900.
  • Avizienyte E, Keppler M, Sandilands E, Brunton VG, Winder SJ, Ng T & Frame MC (2007) . EXP CELL RES, 313(15), 3175-3188.
  • Anderson C, Winder SJ & Borycki A-G (2007) . Dev Dyn, 236(9), 2627-2635.
  • Rezniczek GA, Konieczny P, Nikolic B, Reipert S, Schneller D, Abrahamsberg C, Davies KE, Winder SJ & Wiche G (2007) Plectin 1f scaffolding at the sarcolemma of dystrophic (mdx) muscle fibers through multiple interactions with beta-dystroglycan. J CELL BIOL, 176(7), 965-977.
  • Hnia K, Zouiten D, Cantel S, Chazalette D, Hugon G, Fehrentz JA, Masmoudi A, Diment A, Bramham J, Mornet D & Winder SJ (2007) . BIOCHEM J, 401, 667-677.
  • Batchelor CL, Higginson JR, Chen Y-J, Vanni C, Eva A & Winder SJ (2007) . Cell Cycle, 6(3), 353-363.
  • Lin N, Chen A, Mackley JR, Winder SJ & Yokota H (2007) . AIP Conference Proceedings, 953, 122-131.
  • Mackley JR, Ando J, Herzyk P & Winder SJ (2006) . Biochem J, 396(2), 307-316.
  • Batchelor CL & Winder SJ (2006) . Trends Cell Biol, 16(4), 198-205.
  • Thompson O, Winder S & Peckham M (2006) . The FASEB Journal, 20(5), lb3-lb3.
  • Higginson JR & Winder SJ (2005) . Biochem Soc Trans, 33(Pt 6), 1254-1255.
  • Winder SJ & Ayscough KR (2005) . J Cell Sci, 118(Pt 4), 651-654.
  • Broderick MJF & Winder SJ (2005) . Adv Protein Chem, 70, 203-246.
  • Winder SJ (2004) . Biochem J, 384(Pt 1), e1-e2.
  • Ayscough KR & Winder SJ (2004) . EMBO Rep, 5(10), 947-952.
  • Spence HJ, Chen YJ, Batchelor CL, Higginson JR, Suila H, Carpen O & Winder SJ (2004) . HUM MOL GENET, 13(15), 1657-1668.
  • Spence HJ, Dhillon AS, James M & Winder SJ (2004) . EMBO REP, 5(5), 484-489.
  • Gourlay CW, Carpp LN, Timpson P, Winder SJ & Ayscough KR (2004) . J Cell Biol, 164(6), 803-809.
  • Chen YJ, Spence HJ, Cameron JM, Jess T, Ilsley JL & Winder SJ (2003) Direct interaction of beta-dystroglycan with F-actin. BIOCHEM J, 375, 329-337.
  • Winder SJ, Jess T & Ayscough KR (2003) . Biochem J, 375(Pt 2), 287-295.
  • Winder SJ & Marston G () . PLoS Currents.
  • García‐Aguirre I, Alamillo‐Iniesta A, Rodríguez‐Pérez R, Vélez‐Aguilera G, Amaro‐Encarnación E, Jiménez‐Gutiérrez E, Vásquez‐Limeta A, Samuel Laredo‐Cisneros M, Morales‐Lázaro SL, Tiburcio‐Félix R , Ortega A et al () . Aging Cell.

Book chapters

  • Gimona M & Winder SJ () Wiley-VCH Verlag GmbH & Co. KGaA
  • Gimona M & Winder SJ () , Modular Protein Domains (pp. 321-336). Wiley-VCH Verlag GmbH & Co. KGaA

Conference proceedings

  • Aguilera GV, Lopez JG, Gutierrez EJ, Limeta AV, Cisneros ML, Winder SJ & Cisneros-Vega B (2016) CRM1 mediates nuclear export of beta dystroglycan to maintain correct nuclear envelope integrity and function.. MOLECULAR BIOLOGY OF THE CELL, Vol. 27
  • Cerecedo DA, Martinez-Vieyra I, Maldonado DG, Hernandez EG & Winder SJ (2015) Association of membrane/lipid rafts with the platelet cytoskeleton and the Caveolin PY14 and its Participation in the Adhesion Process. MOLECULAR BIOLOGY OF THE CELL, Vol. 26
  • Martinez-Zarate A, Martinez-Vieyra I, Alonso-Rangel L, Cisneros B, Winder SJ & Cerecedo D (2014) Dystroglycan depletion inhibits the functions of differentiated HL-60 cells.. MOLECULAR BIOLOGY OF THE CELL, Vol. 25
  • Moore C, Mitchell A, La Riviere T, Lipscombe L, Piggott R, Parkin C, Miller G & Winder SJ (2011) . NEUROMUSCULAR DISORDERS, Vol. 21(9-10) (pp 639-639)
  • Miller G, Moore C, Lipscomb L, Mitchell A & Winder S (2010) . Neuromuscular Disorders, Vol. 20 (pp S13-S13)
  • Cross SS, Hollingbury FE, Lippitt JM, Evans CA, Balasubramanian SP, Reed MWR, Handy FC & Winder SJ (2005) Loss of intercellular expression of beta-dystroglycan is a common event in breast cancer. JOURNAL OF PATHOLOGY, Vol. 205 (pp 8-8)
  • Cross SS, Hollingbury FE, Lippitt JM, Feeley KM, Azzouzi AR, Winder SJ & Hamdy FC (2005) Loss of intercellular expression of beta-dystroglycan is a common event in prostate cancer and is significantly associated with loss of differentiation. JOURNAL OF PATHOLOGY, Vol. 205 (pp 15-15)
  • Cross SS, Hollingbury FE, Lippitt JM, Catto JW, Hamdy EC & Winder SJ (2005) beta-dystroglycan is constitutively expressed at the intercellular junctions of epithelial cells and this expression is frequently absent in common cancers. JOURNAL OF PATHOLOGY, Vol. 205 (pp 6-6)
  • Batchelor CL & Winder SJ (2004) ERM-dependent regulation of the actin cytoskeleton by beta-dystroglycan. MOLECULAR BIOLOGY OF THE CELL, Vol. 15 (pp 171A-171A)

Datasets

  • Winder S .
Teaching activities

Level 1

  • BMS109 Cell Biology
  • BMS110 Research Topics in Biomedicine

Level 3

  • Practical and Dissertation Modules

Level 4/Masters (MSc)

  • BMS402 Laboratory Research Project (MBiomedSci)
  • BMS6052 Laboratory Research Project (MSc)
Professional activities and memberships
  • Member of the Scientific Advisory Board for Duchenne UK
  • Member of the TREAT NMD Advisory Committee on Therapeutics (TACT)
  • Member of Editorial Boards of: International Journal of Cell Biology, BioMed Research International, PLoS Currents Muscular Dystrophy, Investigación en Discapacidad, Protein Modules Consortium.
  • External examiner for Molecular Medicine BSc, School of Biological Sciences, University of Edinburgh, Quinquennial review of MSc in Molecular Medicine, School of Biological Sciences, UEA, External Advisory Board for Biomedical Science, International Islamic University Malaysia, External Advisor to the Board of Studies for Biochemistry, Ramnarain Ruia College, Mumbai,
  • Recent Invited Conference presentations: 220th ENMC Workshop on ‘Dystroglycan and the dystroglycanopathies’, Naarden, Holland. EMBO Workshop on ‘The modularity of signalling proteins and networks’, Seefeld, Austria. IIUM Zebrafish Workshop, Kuantan, Malaysia. Action Duchenne International Conference, London. 5th International Workshop for Glycosylation Defects in Muscular Dystrophies, Charlotte, NC, USA.
  • Member of British Society for Cell Biology and Biochemical Society