Professor Stephen A Rolfe
School of Biosciences
Professor of Plant and Microbial Science
Full contact details
School of Biosciences
D65
Alfred Denny Building
Western Bank
Sheffield
S10 2TN
- Profile
-
- BSc (1984) Imperial College of Science and Technology, London
- PhD (1988) University of Cambridge
- European Molecular Biology Fellow, University of California Los Angeles (1988-1991)
- Lecturer, Dept. Animal and Plant Sciences, University of Sheffield (1991-2003)
- Senior Lecturer, School of Biosciences, University of Sheffield (2004-2022)
- Director of Teaching, Dept. Animal and Plant Sciences, University of Sheffield (2008-2015)
- Professor, Chair in Plant Science, School of Biosciences, University of Sheffield (2022-present)
- Research interests
-
My research focuses on plant responses to biotic and abiotic stresses using biological imaging techniques. I lead the P3 Wolfson Centre for Plant Disease Phenomics where we use high-throughput imaging to explore quantitative resistance to plant diseases. We use chlorophyll fluorescence and multi-spectral imaging to quantify disease development and the impact on host plants.
Current projects are focussed on diseases of Brassicas including clubroot and light leaf spot. My group also looks at microbial communities in natural environments using metagenomic approaches. This includes plant pathogen populations but also the root microbiome and degradation of xenobiotics in contaminated environments. Work in my group is funded by the BBSRC, the Wolfson Foundation, Bayer Crop Sciences, Shell Aviation and Airbus.
- Publications
-
Show: Featured publications All publications
Featured publications
Journal articles
- . Journal of Hazardous Materials, 388.
- . The ISME Journal, 13, 1647-1658.
- . Nature Communications, 10(1).
- . The Plant Journal, 97(4), 715-729.
- . The Plant Cell, 30(12), 3058-3073.
- . BMC Genomics, 17.
- . European Journal of Plant Pathology, 145(3), 543-557.
- . Bioresource Technology, 200, 426-434.
- . Ecological Engineering, 74, 290-301.
- . Applied Geochemistry, 48, 28-40.
- . Microbial Ecology, 66(1), 84-95.
- . Journal of Experimental Botany, 64(2), 385-390.
- . Plant J, 71(2), 226-238.
- . Plant Journal, 71(2), 226-238.
- . Protoplasma, 247(3-4), 163-175.
- . Environ Microbiol, 12(9), 2496-2507.
- . FEMS Microbiol Ecol, 71(2), 247-259.
- . FUNCT PLANT BIOL, 36(10-11), 880-892.
- . ENVIRON EXP BOT, 67(1), 101-111.
- . PLANT J, 59(2), 329-343.
- . J PLANT GROWTH REGUL, 28(3), 229-244.
- . J Exp Bot, 57(1), 55-69.
- . New Phytol, 167(3), 881-896.
- . J Exp Bot, 54(389), 1813-1820.
- . Plant Physiol, 131(2), 472-481.
- . Photosynth Res, 72(1), 107-115.
- The base of the leaf acts as a localized sink for photosynthate in mature barley leaves. NEW PHYTOL, 148(2), 257-266.
- . Mol Plant Pathol, 1(2), 99-113.
- Changes in carbon allocation and expression of carbon transporter genes in Betula pendula Roth. colonized by the ectomycorrhizal fungus Paxillus involutus (Batsch) Fr.. PLANT CELL ENVIRON, 23(1), 39-49.
- The effect of Albugo candida (white blister rust) on the photosynthetic and carbohydrate metabolism of leaves of Arabidopsis thaliana. PLANT CELL ENVIRON, 19(8), 967-975.
- Photosynthesis in localised regions of oat leaves infected with crown rust (Puccinia coronata): Quantitative imaging of chlorophyll fluorescence. PLANTA, 199(4), 573-582.
- . Planta, 198(1), 17-23.
- QUANTITATIVE IMAGING OF CHLOROPHYLL FLUORESCENCE. NEW PHYTOL, 131(1), 69-79.
All publications
Books
- . Cambridge University Press.
- . Cambridge University Press.
Journal articles
- . Biofouling, 41(3), 265-282.
- . Groundwater Monitoring & Remediation, 44(4), 92-103.
- . Langmuir, 40(7), 3429-3439.
- . Environmental Science and Pollution Research, 31(11), 16150-16163.
- Genes to ‘phenes’ To truly unlock the potential of plants, genomic and molecular advances need to be supported by high-tech plant phenotyping. Biologist, 71(3).
- . Environmental Science and Pollution Research, 29, 1223-1238.
- . Nutrition Bulletin, 46(4), 497-505.
- . Langmuir, 37(32), 9838-9850.
- . Environmental Pollution, 277.
- . Pest Management Science, 77(9), 3900-3909.
- . Mycorrhiza, 31(1), 69-83.
- . Heliyon, 6(11).
- . Journal of Hazardous Materials, 388.
- . Soil and Tillage Research, 198, 104543-104543.
- . Journal of Hazardous Materials, 391.
- . Current Opinion in Microbiology, 49, 73-82.
- . PLoS ONE, 14(7).
- . The ISME Journal, 13, 1647-1658.
- . Nature Communications, 10(1).
- . Plant Methods, 15(1).
- . The Plant Journal, 97(4), 715-729.
- . Plant Methods, 15.
- . The Plant Cell, 30(12), 3058-3073.
- . Agricultural Water Management, 203, 1-8.
- . Journal of Environmental Management, 206, 1028-1038.
- . Plant Journal, 92(6), 981-994.
- . The Plant Journal, 92(1), 147-162.
- . Advanced Science Letters, 23(5), 4623-4626.
- . BMC Genomics, 17.
- . European Journal of Plant Pathology, 145(3), 543-557.
- . Plant Physiology, 170(3), 1655-1674.
- . Bioresource Technology, 200, 426-434.
- . Ecological Engineering, 74, 290-301.
- . Applied Geochemistry, 48, 28-40.
- . Plant Journal, 76(6), 914-929.
- . Microbial Ecology, 66(1), 84-95.
- . Journal of Experimental Botany, 64(2), 385-390.
- . Plant J, 71(2), 226-238.
- . Plant Journal, 71(2), 226-238.
- . PLoS One, 7(10).
- . Protoplasma, 247(3-4), 163-175.
- . Environ Microbiol, 12(9), 2496-2507.
- . FEMS Microbiol Ecol, 71(2), 247-259.
- . FUNCT PLANT BIOL, 36(10-11), 880-892.
- . ENVIRON EXP BOT, 67(1), 101-111.
- . PLANT J, 59(2), 329-343.
- . J PLANT GROWTH REGUL, 28(3), 229-244.
- . Faraday Discussions, 139, 85-103.
- . Journal of Experimental Botany, 57(1), 24-24.
- . J Exp Bot, 57(1), 55-69.
- . New Phytol, 167(3), 881-896.
- . J Exp Bot, 54(389), 1813-1820.
- . Plant Physiol, 131(2), 472-481.
- . Photosynth Res, 72(1), 107-115.
- . The Plant Journal, 30(6), 691-698.
- The base of the leaf acts as a localized sink for photosynthate in mature barley leaves. NEW PHYTOL, 148(2), 257-266.
- . Mol Plant Pathol, 1(2), 99-113.
- Changes in carbon allocation and expression of carbon transporter genes in Betula pendula Roth. colonized by the ectomycorrhizal fungus Paxillus involutus (Batsch) Fr.. PLANT CELL ENVIRON, 23(1), 39-49.
- The effect of Albugo candida (white blister rust) on the photosynthetic and carbohydrate metabolism of leaves of Arabidopsis thaliana. PLANT CELL ENVIRON, 19(8), 967-975.
- . Planta, 200(2).
- Photosynthesis in localised regions of oat leaves infected with crown rust (Puccinia coronata): Quantitative imaging of chlorophyll fluorescence. PLANTA, 199(4), 573-582.
- . Planta, 198(1), 17-23.
- QUANTITATIVE IMAGING OF CHLOROPHYLL FLUORESCENCE. NEW PHYTOL, 131(1), 69-79.
- . Proceedings of the National Academy of Sciences, 88(7), 2683-2686.
- . Plant Molecular Biology, 15(1), 49-58.
- . Biochimica et Biophysica Acta (BBA) - Bioenergetics, 973(2), 220-226.
- . Biochimica et Biophysica Acta (BBA) - Bioenergetics, 894(3), 434-442.
- , 259-273.
- . Frontiers in Horticulture, 4.
Book chapters
- , Springer Protocols Handbooks (pp. 11-64). Springer Berlin Heidelberg
- , The Physiology of Flowering Plants (pp. 246-269). Cambridge University Press
- , The Physiology of Flowering Plants (pp. 161-176). Cambridge University Press
- , The Physiology of Flowering Plants (pp. 205-220). Cambridge University Press
- , The Physiology of Flowering Plants (pp. 133-158). Cambridge University Press
- , The Physiology of Flowering Plants (pp. 344-372). Cambridge University Press
- , The Physiology of Flowering Plants (pp. 1-6). Cambridge University Press
- , The Physiology of Flowering Plants (pp. 177-204). Cambridge University Press
- , The Physiology of Flowering Plants (pp. 9-59). Cambridge University Press
- , The Physiology of Flowering Plants (pp. 318-343). Cambridge University Press
- , The Physiology of Flowering Plants (pp. 60-99). Cambridge University Press
- , The Physiology of Flowering Plants (pp. 100-132). Cambridge University Press
- , The Physiology of Flowering Plants (pp. 270-317). Cambridge University Press
- , The Physiology of Flowering Plants (pp. 221-245). Cambridge University Press
- (pp. 373-375). Cambridge University Press
- , Plant Molecular Biology 2 (pp. 509-517). Springer US
- , Phytochrome Properties and Biological Action (pp. 167-179). Springer Berlin Heidelberg
Conference proceedings
- . Molecular Plant-Microbe Interactions, Vol. 32(10S) (pp 26-26). Glasgow, Scotland, 14 July 2019 - 14 July 2019.
- . Molecular Plant-Microbe Interactions, Vol. 32(10) (pp S1.32-S1.32). Glasgow, Scotland, 14 July 2019 - 14 July 2019.
- The impacts of quantitative disease resistance on downy mildew epidemiology in mixed host populations. MOLECULAR PLANT-MICROBE INTERACTIONS, Vol. 32(10) (pp 19-19)
- How will novel fuels and materials impact microbial contamination in aircraft fuel systems?. Iash 2015 14th International Symposium on Stability Handling and Use of Liquid Fuels
- The study of cells using scanning force microscopy. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, Vol. 40 (pp 149-149)
- SFM study of bacterial cell walls. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, Vol. 40 (pp 151-151)
- Interactions at cell-mineral interface. Goldschmidt 2010: Earth, Energy, and the Environment (pp A880-A880). Knoxville, Tennessee, USA
- Multi-factorial analysis of surface interactions in single species environmental bacteria and model surfaces. GEOCHIMICA ET COSMOCHIMICA ACTA, Vol. 73(13) (pp A44-A44)
- Mineral-specific attachment of sulphate-reducing bacterial consortia: combined experimental and xDLVO modelling approach. GEOCHIMICA ET COSMOCHIMICA ACTA, Vol. 73(13) (pp A76-A76)
- A comparison of the carbohydrate composition and kinetic properties of sucrose phosphate synthase (SPS) in transgenic tobacco (Nicotiana tabacum) leaves expressing maize SPS protein with untransformed controls. ANNALS OF APPLIED BIOLOGY, Vol. 138(1) (pp 47-55)
Preprints
- , Cold Spring Harbor Laboratory.
- , Springer Science and Business Media LLC.
- , Cold Spring Harbor Laboratory.
- Research group
-
- Luke Cartwright
- Elisa Clagnan
- Juan Mujica
- Raisa Raisa
- Christina Paparokidou
- Sarah Sommer
- Fokion Chatziavgerinos
- Teaching activities
-
Dr Stephen Rolfe won the Departmental Teacher of Year award in 2007.
At level 1, I am module coordinator for Skills for Biologists 1 with responsibility for the IT aspects of the course.
At level 2 I teach Plant, Cell & Environment and Biotechnology and Food. These modules reflect my long–standing interest in plant molecular biology, physiology and biochemistry and cover everything from the perception of light by plants through to the adoption of GM crops in modern agriculture. This theme continues in level 3 where I teach Environmental Regulation in Plants.
In recent years my research has expanded to consider environmental microbiology, particularly contaminated environments. I teach the module Life in Extreme Environments where I cover everything from life in hot springs, deep sea vents, other planets (maybe…) to contaminated aquifers in Wolverhampton! I am also very interested in the ethics of biology and how this affects wider society. The module Biology and Ethics covers a wide range of ethical areas from the impact of advances in genomic sequencing on individuals rights through to patents and Intellectual Property Rights and how they affect developments in agriculture. The aim of this course is to introduce students to key ethical issues but then let them explore topics that are of particular interest or relevance to them.
I have supervised a number of level 4 MBiolSci students – for example last year Joanna Scales examined how clubroot, a disease of Brassicas, alters developmental pathways in its host.
- Professional activities and memberships
-
- External examiner Reading University (2014-2016)
- External examiner SRUC (Scotland's Rural College), Edinburgh, UK (2016-present)