Dr John Rafferty

School of Biosciences

Reader in Structural Biology

Principal Admissions Tutor

j.rafferty@sheffield.ac.uk
Firth Court

Full contact details

Dr John Rafferty
School of Biosciences
Firth Court
Western Bank
Sheffield
S10 2TN
Profile

Career history

  • 2005 - present: Reader in Structural Biology, School of Biosciences, Sheffield Univ
  • 1999 - 2004: Royal Society Olga Kennard Fellow, Mol.Biol.&Biotech.Dept, Sheffield Univ
  • 1996 - 1999: BBSRC David Phillips Research Fellow, Mol.Biol.&Biotech.Dept, Sheffield Univ.
  • 1992 - 1996: PDRA, Mol.Biol. & Biotech. Dept, Sheffield Univ.
  • 1990 - 1991: PDRA, Biochem. & Mol.Biol. Dept, Leeds Univ.
  • 1989 - 1990: PDRA, Biophysics Dept, Leeds Univ.
  • 1986 - 1990: PhD, Biophysics Dept, Leeds Univ.
Research interests

Structural Biology

Publications

Show: Featured publications All publications

Journal articles

  • Phansopa C, Roy S, Rafferty JB, Douglas CWI, Pandhal J, Wright PC, Kelly DJ & Stafford GP (2014) . Biochem J, 458(3), 499-511.
  • Green V, Curtis FA, Sedelnikova S, Rafferty JB & Sharples GJ (2013) . Mol Microbiol, 89(6), 1240-1258.
  • Afonso JP, Chintakayala K, Suwannachart C, Sedelnikova S, Giles K, Hoyes JB, Soultanas P, Rafferty JB & Oldham NJ (2013) . Nucleic Acids Res, 41(9), 5115-5126.
  • Salmon RC, Cliff MJ, Rafferty JB & Kelly DJ (2013) . PLoS One, 8(3), e59844.
  • Canas C, Carrasco B, Garcia-Tirado E, Rafferty JB, Alonso JC & Ayora S (2011) . J MOL BIOL, 410(1), 39-49.
  • Kale A, Phansopa C, Suwannachart C, Craven CJ, Rafferty JB & Kelly DJ (2011) . J Biol Chem, 286(24), 21254-21265.
  • Roujeinikova A, Simon WJ, Gilroy J, Rice DW, Rafferty JB & Slabas AR (2007) . J Mol Biol, 365(1), 135-145.
  • Macmaster R, Sedelnikova S, Baker PJ, Bolt EL, Lloyd RG & Rafferty JB (2006) . Nucleic Acids Res, 34(19), 5577-5584.
  • Thaw P, Sedelnikova SE, Muranova T, Wiese S, Ayora S, Alonso JC, Brinkman AB, Akerboom J, van der Oost J & Rafferty JB (2006) . Nucleic Acids Res, 34(5), 1439-1449.
  • Kehoe LE, Snidwongse J, Courvalin P, Rafferty JB & Murray IA (2003) . J Biol Chem, 278(32), 29963-29970.
  • Bailey S, Sedelnikova SE, Mesa P, Ayora S, Waltho JP, Ashcroft AE, Baron AJ, Alonso JC & Rafferty JB (2003) . J Biol Chem, 278(17), 15304-15312.
  • Rafferty JB, Bolt EL, Muranova TA, Sedelnikova SE, Leonard P, Pasquo A, Baker PJ, Rice DW, Sharples GJ & Lloyd RG (2003) . Structure, 11(12), 1557-1567.
  • Abdelghany HM, Bailey S, Blackburn GM, Rafferty JB & McLennan AG (2003) . J BIOL CHEM, 278(7), 4435-4439.

All publications

Journal articles

  • Alrafaie AM, Pyrzanowska K, Smith EM, Partridge DG, Rafferty J, Mesnage S, Shepherd J & Stafford GP (2024) . Virus Research, 347.
  • Curry E, Sedelnikova S, Rafferty J, Hulley M, Pohle M, Muir G & Brown A (2024) . Biotechnology Journal, 19(6).
  • Kaushik A, Than T, Petela NJ, Voulgaris M, Percival C, Daniels P, Rafferty JB, Nasmyth KA & Hu B (2023) . Nature Communications, 14(1).
  • Satur MJ, Urbanowicz PA, Spencer DIR, Rafferty J & Stafford GP (2022) . Biochemical Journal, 479(17), 1785-1806.
  • Bisson C, Salmon RC, West L, Rafferty JB, Hitchcock A, Thomas GH & Kelly DJ (2022) . The FEBS Journal, 289(2), 436-456.
  • Bennison DJ, Nakamoto JA, Craggs TD, Milón P, Rafferty JB & Corrigan RM (2021) . mBio, 12(6).
  • Churchill-Angus AM, Schofield THB, Marlow TR, Sedelnikova SE, Wilson JS, Rafferty JB & Baker PJ (2021) . Scientific Reports, 11(1).
  • Wilson JS, Churchill-Angus AM, Davies SP, Sedelnikova SE, Tzokov SB, Rafferty JB, Bullough PA, Bisson C & Baker PJ (2019) . Nature Communications, 10(1).
  • Ji S, Dix SR, Aziz AA, Sedelnikova SE, Baker PJ, Rafferty JB, Bullough PA, Tzokov SB, Agirre J, Li F-L & Rice DW (2019) . Journal of Biological Chemistry, 294(48), 18077-18091.
  • Rosa LT, Dix SR, Rafferty JB & Kelly DJ (2019) . Journal of Molecular Biology, 431(2), 351-367.
  • Rosa LT, Dix SR, Rafferty JB & Kelly DJ (2017) . FEBS Journal.
  • Khavnekar S, Dantu SC, Sedelnikova S, Ayora S, Rafferty J & Kale A (2017) . Nucleic Acids Research, 45(2), 975-986.
  • AlMalki F, Flemming C, Zhang J, Feng M, Sedelnikova S, Ceska T, Rafferty J, Sayers JR & Artymiuk P (2016) . Nature Structural and Molecular Biology, 23, 640-646.
  • Phansopa C, Roy S, Rafferty JB, Douglas CWI, Pandhal J, Wright PC, Kelly DJ & Stafford GP (2014) . Biochem J, 458(3), 499-511.
  • Green V, Curtis FA, Sedelnikova S, Rafferty JB & Sharples GJ (2013) . Mol Microbiol, 89(6), 1240-1258.
  • Afonso JP, Chintakayala K, Suwannachart C, Sedelnikova S, Giles K, Hoyes JB, Soultanas P, Rafferty JB & Oldham NJ (2013) . Nucleic Acids Res, 41(9), 5115-5126.
  • Salmon RC, Cliff MJ, Rafferty JB & Kelly DJ (2013) . PLoS One, 8(3), e59844.
  • Abd Aziz AG, Sedelnikova SE, Ruzheinikov SN, Thorpe S, Mohamed R, Nathan S, Rafferty JB, Baker PJ & Rice DW (2012) . Acta Crystallogr Sect F Struct Biol Cryst Commun, 68(Pt 8), 917-922.
  • Fahy E, Carroll J, Rafferty J, Roantree V, Reid C, Norman M & Clarke S (2011) . Biology & Environment: Proceedings of the Royal Irish Academy, 110(2), 95-108.
  • Canas C, Carrasco B, Garcia-Tirado E, Rafferty JB, Alonso JC & Ayora S (2011) . J MOL BIOL, 410(1), 39-49.
  • Kale A, Phansopa C, Suwannachart C, Craven CJ, Rafferty JB & Kelly DJ (2011) . J Biol Chem, 286(24), 21254-21265.
  • Muench SP, Prigge ST, McLeod R, Rafferty JB, Kirisits MJ, Roberts CW, Mui EJ & Rice DW (2007) . Acta Crystallogr D Biol Crystallogr, 63(Pt 3), 328-338.
  • Roujeinikova A, Simon WJ, Gilroy J, Rice DW, Rafferty JB & Slabas AR (2007) . J Mol Biol, 365(1), 135-145.
  • Macmaster R, Sedelnikova S, Baker PJ, Bolt EL, Lloyd RG & Rafferty JB (2006) . Nucleic Acids Res, 34(19), 5577-5584.
  • Thaw P, Sedelnikova SE, Muranova T, Wiese S, Ayora S, Alonso JC, Brinkman AB, Akerboom J, van der Oost J & Rafferty JB (2006) . Nucleic Acids Res, 34(5), 1439-1449.
  • McGregor N, Ayora S, Sedelnikova S, Carrasco B, Alonso JC, Thaw P & Rafferty J (2005) . Structure, 13(9), 1341-1351.
  • Dickman MJ, Sedelnikova SE, Rafferty JB & Hornby DP (2004) . J Biochem Biophys Methods, 58(1), 39-48.
  • Muranova TA, Sedelnikova SE, Leonard PM, Pasquo A, Bolt EL, Lloyd RG & Rafferty JB (2003) . Acta Crystallogr D Biol Crystallogr, 59(Pt 12), 2262-2264.
  • Muench SP, Rafferty JB, McLeod R, Rice DW & Prigge ST (2003) . Acta Crystallogr D Biol Crystallogr, 59(Pt 7), 1246-1248.
  • Kehoe LE, Snidwongse J, Courvalin P, Rafferty JB & Murray IA (2003) . J Biol Chem, 278(32), 29963-29970.
  • Bailey S, Sedelnikova SE, Mesa P, Ayora S, Alonso JC & Rafferty JB (2003) . Acta Crystallogr D Biol Crystallogr, 59(Pt 6), 1090-1092.
  • Bailey S, Sedelnikova SE, Mesa P, Ayora S, Waltho JP, Ashcroft AE, Baron AJ, Alonso JC & Rafferty JB (2003) . J Biol Chem, 278(17), 15304-15312.
  • Santos-Sierra S, Lemonnier M, Nuñez B, Hargreaves D, Rafferty J, Giraldo R, Andreu JM & Dı́az-Orejas R (2003) . Plasmid, 50(2), 120-130.
  • Rafferty JB, Bolt EL, Muranova TA, Sedelnikova SE, Leonard P, Pasquo A, Baker PJ, Rice DW, Sharples GJ & Lloyd RG (2003) . Structure, 11(12), 1557-1567.
  • Abdelghany HM, Bailey S, Blackburn GM, Rafferty JB & McLennan AG (2003) . J BIOL CHEM, 278(7), 4435-4439.
  • Dickman MJ, Ingleston SM, Sedelnikova SE, Rafferty JB, Lloyd RG, Grasby JA & Hornby DP (2002) . Eur J Biochem, 269(22), 5492-5501.
  • Hargreaves D, Santos-Sierra S, Giraldo R, Sabariegos-Jareño R, de la Cueva-Méndez G, Boelens R, Díaz-Orejas R & Rafferty JB (2002) . Structure, 10(10), 1425-1433.
  • Slabas AR, Kroon JTM, Scheirer TP, Gilroy JS, Hayman M, Rice DW, Turnbull AP, Rafferty JB, Fawcett T & Simon WJ (2002) . J BIOL CHEM, 277(46), 43918-43923.
  • Ettema TJG, Brinkman AB, Tani TH, Rafferty JB & van der Oost J (2002) . J BIOL CHEM, 277(40), 37464-37468.
  • Roujeinikova A, Baldock C, Simon WJ, Gilroy J, Baker PJ, Stuitje AR, Rice DW, Slabas AR & Rafferty JB (2002) . Structure, 10(6), 825-835.
  • Bailey S, Sedelnikova SE, Blackburn GM, Abdelghany HM, Baker PJ, McLennan AG & Rafferty JB (2002) . Structure, 10(4), 589-600.
  • Bailey S, Sedelnikova SE, Blackburn GM, Abdelghany HM, McLennan AG & Rafferty JB (2002) . Acta Crystallogr D Biol Crystallogr, 58(Pt 3), 526-528.
  • Roujeinikova A, Baldock C, Simon WJ, Gilroy J, Baker PJ, Stuitje AR, Rice DW, Rafferty JB & Slabas AR (2002) . Acta Crystallogr D Biol Crystallogr, 58(Pt 2), 330-332.
  • Hargreaves D, Giraldo R, Santos-Sierra S, Boelens R, Rice DW, Díaz Orejas R & Rafferty JB (2002) . Acta Crystallogr D Biol Crystallogr, 58(Pt 2), 355-358.
  • Abdelghany HM, Gasmi L, Cartwright JL, Bailey S, Rafferty JB & McLennan AG (2001) Cloning, characterisation and crystallisation of a diadenosine 5 ',5 '''-P-1,P-4-tetraphosphate pyrophosphohydrolase from Caenorhabditis elegans. BBA-PROTEIN STRUCT M, 1550(1), 27-36.
  • Showell C, Rafferty JB & Cunliffe VT (2001) Functional studies of the Xenopus Polycomb-group proteins Xeed and XEz.. DEV BIOL, 235(1), 262-263.
  • Sedelnikova SE, Smits SH, Leonard PM, Brinkman AB, van der Oost J, Rafferty JB & Rice DW (2001) . Acta Crystallogr D Biol Crystallogr, 57(Pt 6), 886-888.
  • Levy CW, Baldock C, Wallace AJ, Sedelnikova S, Viner RC, Clough JM, Stuitje AR, Slabas AR, Rice DW & Rafferty JB (2001) . J Mol Biol, 309(1), 171-180.
  • Turnbull AP, Rafferty JB, Sedelnikova SE, Slabas AR, Schierer TP, Kroon JT, Nishida I, Murata N, Simon JW & Rice DW (2001) . Acta Crystallogr D Biol Crystallogr, 57(Pt 3), 451-453.
  • Leonard PM, Smits SH, Sedelnikova SE, Brinkman AB, de Vos WM, van der Oost J, Rice DW & Rafferty JB (2001) . EMBO J, 20(5), 990-997.
  • McLeod R, Muench SP, Rafferty JB, Kyle DE, Mui EJ, Kirisits MJ, Mack DG, Roberts CW, Samuel BU, Lyons RE , Dorris M et al (2001) Triclosan inhibits the growth of Plasmodium falciparum and Toxoplasma gondii by inhibition of apicomplexan Fab I. INT J PARASITOL, 31(2), 109-113.
  • Turnbull AP, Rafferty JB, Sedelnikova SE, Slabas AR, Schierer TP, Kroon JT, Simon JW, Fawcett T, Nishida I, Murata N & Rice DW (2001) . Structure, 9(5), 347-353.
  • Slabas AR, Simon WR, Schierer T, Kroon J, Fawcett T, Hayman M, Gilroy J, Nishida I, Murata N, Rafferty J , Turnbull A et al (2000) . Biochemical Society Transactions, 28(6), 677-679.
  • Hayman MW, Fawcett T, Schierer TF, Simon JW, Kroon JTM, Gilroy JS, Rice DW, Rafferty J, Turnbull AP, Sedelnikova SE & Slabas AR (2000) . Biochemical Society Transactions, 28(6), 680-681.
  • Hayman MW, Fawcett T, Schierer TF, Simon JW, Kroon JTM, Gilroy JS, Rice DW, Rafferty J, Turnbull AP, Sedelnikova SE & Slabas AR (2000) . Biochemical Society Transactions, 28(6), 680-680.
  • Slabas AR, Simon WR, Schierer T, Kroon J, Fawcett T, Hayman M, Gilroy J, Nishida I, Murata N, Rafferty J , Turnbull A et al (2000) . Biochemical Society Transactions, 28(6), 677-677.
  • Fisher M, Kroon JT, Martindale W, Stuitje AR, Slabas AR & Rafferty JB (2000) . Structure, 8(4), 339-347.
  • Fisher M, Sedelnikova SE, Martindale W, Thomas NC, Simon JW, Slabas AR & Rafferty JB (2000) . Acta Crystallogr D Biol Crystallogr, 56(Pt 1), 86-88.
  • de Boer GJ, Pielage GJA, Nijkamp HJJ, Slabas AR, Rafferty JB, Baldock C, Rice DW & Stuitje AR (1999) Molecular genetic analysis of enoyl-acyl carrier protein reductase inhibition by diazaborine. MOL MICROBIOL, 31(2), 443-450.
  • Roujeinikova A, Levy CW, Rowsell S, Sedelnikova S, Baker PJ, Minshull CA, Mistry A, Colls JG, Camble R, Stuitje AR , Slabas AR et al (1999) . J Mol Biol, 294(2), 527-535.
  • Roujeinikova A, Sedelnikova S, de Boer GJ, Stuitje AR, Slabas AR, Rafferty JB & Rice DW (1999) . J Biol Chem, 274(43), 30811-30817.
  • Levy CW, Roujeinikova A, Sedelnikova S, Baker PJ, Stuitje AR, Slabas AR, Rice DW & Rafferty JB (1999) Molecular basis of triclosan activity. NATURE, 398(6726), 383-384.
  • Hargreaves D, Rafferty JB, Sedelnikova SE, Lloyd RG & Rice DW (1999) . Acta Crystallogr D Biol Crystallogr, 55(Pt 1), 263-265.
  • Baldock C, Rafferty JB, Stuitje AR, Slabas AR & Rice DW (1998) . J Mol Biol, 284(5), 1529-1546.
  • Hargreaves D, Rice DW, Sedelnikova SE, Artymiuk PJ, Lloyd RG & Rafferty JB (1998) . Nat Struct Biol, 5(6), 441-446.
  • Baldock C, de Boer GJ, Rafferty JB, Stuitje AR & Rice DW (1998) . Biochem Pharmacol, 55(10), 1541-1549.
  • Rafferty JB, Fisher M, Langridge SJ, Martindale W, Thomas NC, Simon JW, Bithell S, Slabas AR & Rice DW (1998) . Acta Crystallogr D Biol Crystallogr, 54(Pt 3), 427-429.
  • Rafferty JB, Ingleston SM, Hargreaves D, Artymiuk PJ, Sharples GJ, Lloyd RG & Rice DW (1998) . J Mol Biol, 278(1), 105-116.
  • Rice DW, Rafferty JB, Artymiuk PJ & Lloyd RG (1997) . Curr Opin Struct Biol, 7(6), 798-803.
  • Sedelnikova SE, Rafferty JB, Hargreaves D, Mahdi AA, Lloyd RG & Rice DW (1997) . Acta Crystallogr D Biol Crystallogr, 53(Pt 1), 122-124.
  • Baldock C, Rafferty JB, Sedelnikova SE, Baker PJ, Stuitje AR, Slabas AR, Hawkes TR & Rice DW (1996) . Science, 274(5295), 2107-2110.
  • Slabas AR, Brown AP, Rafferty JB, Rice DW, Baldock C, Kroon JTM, Simon W, Stuitje AR & Brough CL (1996) Soluble and membrane bound components of plant lipid synthesis. CR ACAD SCI III-VIE, 319(11), 1043-1047.
  • Baldock C, Rafferty JB, Sedelnikova SE, Bithell S, Stuitje AR, Slabas AR & Rice DW (1996) . Acta Crystallogr D Biol Crystallogr, 52(Pt 6), 1181-1184.
  • Rafferty JB, Sedelnikova SE, Hargreaves D, Artymiuk PJ, Baker PJ, Sharples GJ, Mahdi AA, Lloyd RG & Rice DW (1996) . Science, 274(5286), 415-421.
  • Rafferty JB, Simon JW, Baldock C, Artymiuk PJ, Baker PJ, Stuitje AR, Slabas AR & Rice DW (1995) . Structure, 3(9), 927-938.
  • SOMERS WS, RAFFERTY JB, PHILLIPS K, STRATHDEE S, HE Y, McNALLY T, MANFIELD I, NAVRATIL O, OLD IG, SAINT‐GIRONS I , STOCKLEY PG et al (1994) . Annals of the New York Academy of Sciences, 726(1), 105-117.
  • Rafferty JB, Simon JW, Stuitje AR, Slabas AR, Fawcett T & Rice DW (1994) . J Mol Biol, 237(2), 240-242.
  • Wright CE, Raffertya JB, Flower DR, Groom CR, Findlay JBC, North ACT, Phillips SEV & Zagalsky PF (1992) . Journal of Molecular Biology, 224(1), 283-284.
  • Phillips SEV, Manfield I, Parsons I, Davidson BE, Rafferty JB, Somers WS, Margarita D, Cohen GN, Saint-Girons I & Stockley PG (1989) . Nature, 341(6244), 711-715.
  • Rafferty JB, Somers WS, Saint-Girons I & Phillips SEV (1989) . Nature, 341(6244), 705-710.
  • Rafferty JB, Phillips SEV, Rojas C, Boulot G, Saint-girons I, Guillou Y & Cohen GN (1988) . Journal of Molecular Biology, 200(1), 217-219.

Book chapters

  • Brown AP, Slabas AR & Rafferty JB (2009) , Advances in Photosynthesis and Respiration (pp. 11-34). Springer Netherlands
  • Simon JW, Gilroy J, Roujenikova A, Rafferty J, Baldock C, Baker PJ, Rice DW, Stuitje AR & Slabas AR (2003) , Advanced Research on Plant Lipids (pp. 139-142). Springer Netherlands
  • Slabas AR, Rafferty J, Rice D, Baldock C, Simon JW, Thomas N, Bithell S & Stuitje AR (1997) , Physiology, Biochemistry and Molecular Biology of Plant Lipids (pp. 38-41). Springer Netherlands
  • Slabas AR, Brough C, Kroon J, Simon W, Swinhoe R, Rice D, Rafferty J, Winz R, Stuitjet AR & Elborough K (1995) , Plant Lipid Metabolism (pp. 55-57). Springer Netherlands

Conference proceedings

  • Roujeinikova A, Simon WJ, Gilroy J, Rice DW, Rafferty JB & Slabas AR (2007) Structures of fatty acyl-( acyl carrier protein) thioesters reveal a binding cavity that expands to fit longer substrates. FEBS JOURNAL, Vol. 274 (pp 223-223)
  • Bailey S, Abdelghany HM, Sedelnikova SE, Blackburn GM, Baker PJ, Rafferty JB & McLennan AG (2002) Crystal structure and mutational analysis of the diadenosine tetraphosphate (Ap4A) 'Nudix' hydrolase from Caenorhabditis elegans. DRUG DEVELOPMENT RESEARCH, Vol. 56(4) (pp 567-567)
  • Slabas AR, Hanley ZAC, Schierer TED, Rice D, Turnbull A, Rafferty J, Simon B & Brown A (2001) . Journal of Plant Physiology, Vol. 158(4) (pp 505-513)
  • Fisher M, Martindale W, Slabas AR, Rice DW & Rafferty JB (1999) CRYSTALLOGRAPHIC STUDIES OF THE NADP-DEPENDENT β-KETO ACP REDUCTASE FROM ESCHERICHIA COLI. ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES, Vol. 55 (pp 293-294)

Preprints

  • Curry E, Sedelnikova S, Rafferty J, Hulley M & Brown A (2024) , Cold Spring Harbor Laboratory.
  • Hu B, Kaushik A, Than T, Petela N, Voulgaris M, Percival C, Daniels P, Rafferty J & Nasmyth K (2022) , Springer Science and Business Media LLC.
  • Bennison DJ, Nakamoto JA, Craggs TD, Milón P, Rafferty JB & Corrigan RM () .
Teaching activities

Level 3 modules

  • MBB344 Genomic Science
  • MBB362 Biochemistry Data Handling

Level 2 modules

  • MBB261 Biochemistry 2
  • MBB265 Practical Molecular Bioscience 2

Level 1 modules

  • MBB161 Biochemistry (Module Coordinator)
  • MBB165 Practical Molecular Bioscience 1
Professional activities and memberships

External examiner Univ. Birmingham